MICROPATTERNED DISHES

Patterned dish ready to be plated with cells

  • Ready to use

    Plate the cells in the petri dish and observe them

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    High quality cell imaging

    Observe the cells through the micropatterned glass substrate

  • Very stable coating

    Can be stored for months thanks to the covalent bond with the glass bottom

The micropatterned petri dishes are 35 mm glass bottom petri dishes printed with micropatterns.
They are ready to use, you just need to plate your cells on the glass substrate and observe them on the specific micropattern.

The 4Dcell micropatterned dishes are provided in sterile individual plastic bags. They are sold by 20 or 50 dishes and you can choose the shape of the micropattern and the anti-adhesive coating for each of them.

The standard shapes offered by 4Dcell are the disks, lines, squares, rectangles, triangles and grids.
You can also ask for a customized shape that we can design for you.

 

4Dcell offers 3 options of covalent coatings:

  • Standard anti-adhesive polymer with the same anti-fouling effect than traditional PLL-g- PEG, but with a more stable bonding to the glass slide (covalent).
  • Oxidation resistance anti-adhesive polymer which is less sensitive to oxidation and has the same type of covalent bonding to the glass slide.
  • Long term cell culture anti-adhesive polymer which is the best in terms of anti-fouling treatment and bonding to the glass slide. Observable by phase contrast microscopy. This is the best option for long term cell culture on micropatterns (more than a week of cell culture).

Standard positionning of micropattern areas

The Micropatterned Dish provides a wide range of applications. Study and measure:

  • Cell morphology
    • Multicellular interactions: tissue assembly and morphogenesis
    • Regulation of cell size, mitosis and meiosis
    • Mechanics of cell Division and cytokinesis
    • Membrane shaping
  • Sub-cellular organisation
    • Mechanical coupling from nucleus to extracellular matrix
    • Border dynamics: envelope organization and remodeling
    • Organelles in metabolism and stress responses
    • Organelle morphogenesis, targeting, and distribution
    • Cytoskeletal and actin dynamics

Video of cells developing on the long term cell culture polymer micropatterns.
Dr Martial Balland, Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier.

line micropatterns

Image of micropatterned cells in lines (labelled by fluorescence) obtained with a 4Dcell photomask
Hôpital Saint Louis, experiment run with Dr. Benoit Vianay