> MICROPATTERNED SLIDES
Patterned slides for immunofluorescence observation
The micropatterned slides are 24 mm glass slides printed with micropatterns.
They are ready to use, you just need to plate your cells on the glass substrate and observe them on the specific micropattern.
> Ready to use
Plate the cells on the slides and observe them
> Adaptable to your imaging system
Place your slide on the imaging system suitable for your study
> Very stable coating
Can be stored for months thanks to the covalent bond with the glass bottom
The 4Dcell micropatterned slides are provided into classic petri dishes in sterile individual plastic bags.
They are sold by 20 or 50 dishes and you can choose the shape of the micropattern and the anti-adhesive coating for each of them.
> MICROPATTERNS SHAPES
The standard shapes offered by 4Dcell are:
disks
lines
triangles
squares
rectangles
grids
Or you can ask for customized shapes.
Standard micropatterns sizes go from 10 µm to 100 µm.
Each type of micropatterned coverslip (disks, rectangles, squares, etc.) is arranged as described in the scheme bellow (e.g.: disks).
In each coverslip, there are seven zones with distinct pattern sizes (ranging from 10 µm to 100 µm). There is an eighth zone where all the sizes are placed together.
You can also ask for a customized shape that we can design for you.
> 4DCELL OFFERS 3 OPTIONS OF COVALENT COATINGS
Standard anti-adhesive polymer with the same anti-fouling effect than traditional PLL-g- PEG, but with a more stable bonding to the glass slide (covalent).
Oxidation resistance anti-adhesive polymer which is less sensitive to oxidation and has the same type of covalent bonding to the glass slide.
Long term cell culture anti-adhesive polymer which is the best in terms of anti-fouling treatment and bonding to the glass slide.
Observable by phase contrast microscopy. This is the best option for long term cell culture on micropatterns (more than a week of cell culture).
Standard positionning of micropattern areas
> CANCER
Migration of cancer cells (line patterns)
Cell shape standardization
> ORGAN PHYSIOLOGY
Migration of somatic and cancer cells
Cell shape standardization
Cardiomyocyte beating properties
Neural network
> RARE DISEASES
Cell nucleus integrity
Nuclear plasticity
Neural network
> FUNDAMENTAL RESEARCH
Cell shape standardization
Standardized connectivity between neurons
> BIOMOLECULAR IN VITRO MODELS
Spatial autoorganization of macromolecules (at the microscale)
Video of cells developing on the long term cell culture polymer micropatterns.
Dr Martial Balland, Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier.
hPSC-CM on line micropatterns of 10 to 70 µm width imaged by fluorescence microscopy.
Dussaud S., Jouve C., Hulot J.S., 2018.
Image of micropatterned HeLa cells in lines of 100 to 10 µm width, 4Dcell lab (2018).
Image of micropatterned cells in lines (labelled by fluorescence) obtained with a 4Dcell photomask.
Hôpital Saint Louis, experiment run with Dr. Benoit Vianay.
