MICROPATTERNS FABRICATION KIT

Make your own adhesive micropatterns

  • Control the conditions of the experiment

    Run your micropatterning experiment in accordance with your research needs

  • Use a simple and affordable method

    Everything is provided to support you in your experiment

  • High quality materials

    Choose the best materials to make homogeneous and reproducible micropatterns

The 4Dcell micropatterning kit includes everything you need to make your own adhesive micropatterns on glass slides for an affordable price.

For each kit, you can choose the shape of the micropatterns among the standard ones offered by 4Dcell or you can customize a specific micropattern.

Which kit fits your needs ?

  • If you never made any micropatterns:
    Micropatterning Station (micropatterning box + UV Printer)
  • If you already have a UV Printer:
    Micropatterning Box (consumables box + optical mask)
  • If you have all the equipment but want to access the best chemicals:
    Consumables Box (slides and aliquots of all the needed solutions)

Each element can also be ordered separately.

Micropatterns Shapes

  • disks
  • lines
  • triangles
  • squares
  • rectangles
  • grids

Or you can ask for customized shapes.

Standard micropatterns sizes go from 10 µm to 100 µm.

What do you need to make your own micropatterns ?

To manufacture 100 micropatterned slides, you need:

  • Optical mask: contains the micropattern features. If you already have optical masks compatible with deep UV, you can use them with our kit
  • 100 glass slides
  • Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution
  • Aliquots of a fluorescent marker to reveal the micropatterns (for example, fluorescently labeled fibrinogen)
  • Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind to the glass slide
  • UV Printer (activation and printing)

We put our expertise at your disposal to help you make your choice.

Micropatterning kits

The Micropattern Fabrication Kit provides a wide range of applications. Study and measure:

CANCER

  • Migration of cancer cells (line patterns)
  • Cell shape standardization

 

ORGAN PHYSIOLOGY

  • Migration of somatic and cancer cells
  • Cell shape standardization
  • Cardiomyocyte beating properties
  • Neural network

 

RARE DISEASES

  • Cell nucleus integrity
  • Nuclear plasticity
  • Neural network

 

FUNDAMENTAL RESEARCH

  • Cell shape standardization
  • Standardized connectivity between neurons

 

BIOMOLECULAR IN VITRO MODELS

  • Spatial autoorganization of macromolecules
    (at the microscale)

Video of cells developing on PNIPAM micropatterns for long term cell culture.
Dr Martial Balland, Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier.

line micropatterns

Image of micropatterned cells in lines (labelled by fluorescence) obtained with a 4Dcell photomask
Hôpital Saint Louis, experiment run with Dr. Benoit Vianay