Lamina assay
Control of nuclear membrane rupture
4DCELL DEVICE
READ-OUTS
Nuclear membrane rupture, nuclear blebs, lamina regulation
STANDARD CULTURE LIMITATION
Repeated rounds of nuclear envelope (NE) rupture and repair have been observed in laminopathy. However, it is an enormous difficulty to reproduce and accurately control such mechanism, while keeping the ability to image and track the cells.
LAMINA ASSAY BENEFITS
Using 4Dcell confiner you can precisely and dynamically control the confinement parameter in cultured cells and acquire high-resolution images.
EXAMPLES
Cell confiner [1]
Quantification of nucelus height U2OS GFP-LmnB1 cells [2]
Height of cells is smaller when cells are confined
Quantification of chromatin hernia formed during 24-h time-lapse imaging [2]
Confinement causes a significant increase in chromatin hernia frequency