Cancer aggressiveness assays
Quantification of contractility of cancer cells
Quantification of contractility of cancer cells
Cell contractility, cell stiffness
Contractility is an important hallmark of cancer aggressivity, but no commercial assay actually allows its quantification.
After confinement of cells between two layers of non-adhesive gels of controlled stiffness, cells round up proportionally to their contractile activity. A relevant index is extracted relying only on the contractile activity of the cell.
Contractility measurement of HeLa cells in between gels with a stiffness of 1 kPa [1].
[1] Liu, Y.-J., et al. Cell. 2015 Feb 12;160(4):659-67