Cancer aggressiveness assays

Quantification of contractility of somatic or cancer cells

4DCELL DEVICE

Cell Confiner

 

READ-OUTS

Cell contractility, cell stiffness

 

STANDARD CULTURE LIMITATION

Contractility is an important hallmark of cancer aggressivity, but no commercial assay actually allows to functionally quantify it.

 

CONTRACTILITY ASSAYS

After confinement of cells in-between 2 layers of non-adhesive gels of controlled stiffness, cells round up proportionally to their contractile activity.  A relevant index is extracted relying  uniquely to the contractile activity of the cell.

 

EXAMPLE

Contractility measurement of HeLa cells in between gels of 1kPa of stiffness [1].

Confinement application

REFERENCES

[1] Liu, Y.-J; et al. (2015). Cell, 160