Cancer aggressiveness assays

Quantification of contractility of cancer cells

4DCELL DEVICE

READ-OUTS

Cell contractility, cell stiffness

STANDARD CULTURE LIMITATION

Contractility is an important hallmark of cancer aggressivity, but no commercial assay actually allows its quantification.

CONTRACTILITY ASSAY BENEFITS

After confinement of cells between two layers of non-adhesive gels of controlled stiffness, cells round up proportionally to their contractile activity. A relevant index is extracted relying only on the contractile activity of the cell.

EXAMPLE

Contractility measurement of HeLa cells in between gels with a stiffness of 1 kPa [1].

REFERENCES

[1] Liu, Y.-J., et al. Cell. 2015 Feb 12;160(4):659-67