Cells are homogeneoulsy confined/compressed between two parallel surfaces with sub-micrometer resolution.
Different confinement heights (eg. 1 µm – 300 µm)
Allows long-term cell culture and cell proliferation to proceed while preserving perfect control of confinement
Compatible with high-resolution optical microscopy systems
Can process a sufficiently large number of cells to allow whole gene expression analyses to be performed
Can be combined with bio-functionalized micro-structured substrates and/or with different matrixes (geometry control)
Can be combined with gels (stiffness control)
Compatible with any cell culture substrate (Petri dishes to 96-well plates)
Cell migration 2.5D, migration and interaction of non-adhesive cells, cell squeezing, imaging of flat cells (organels aligned in 2D), super-resolution video-microscopy (organels move less), contractility assay, etc
HeLa cells: not confined, 5 µm, 3 µm.
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