Mesenchymal stem cells assay
Control cell fate and differentiation
Control cell fate and differentiation
Coverslips coated with microstructured gels
Observation of MSCs fate
Mechanical and biophysical mechanisms regulate mesenchymal stem cells (MSCs) fate decision. However, these mechanisms are not well defined.
Control of MSCs differentiation is needed for many therapies such as regenerative ones [1].
Using 4Dcell gels, with tunable stiffness, cell fate can be chosen depending on the stiffness. In addition, by using 4Dcell microstructured gels and depending on the size area, cells tend to differentiate into adipocytes or osteoblasts.
MSCs differentiation depending of the stiffness [2]
By modifying the geometry and matrix elasticity of the underlying patterns, cells were able to choose lineage commitment based on the physical cues presented.
MSCs differentiation depending of the geometry [3]
By modifying the geometry and matrix elasticity of the underlying patterns, cells were able to choose lineage commitment based on the physical cues presented.
[1] Piroli, M. E. et al. (2018). Annals of biomedical engineering, 46(6), 888-898.
[2] Peng, T., et al. (2017). BMC systems biology, 11(1), 55.
[3] Harris, G. M., et al. (2014). Advanced functional materials, 24(16), 2396-2403.