> COVERSLIPS COATED WITH MICROSTRUCTURED GELS

Control the shape and the stiffness to reproduce the in vivo environment

4Dcell offers microstructured polyacrylamide gel coated coverslips for cell culture.  

The gel based substrates contain soft or rigid microstructures in the form of open microchannels (grooves) or wells.

Therefore, cells can be cultured on substrates that mimic both topographical features and stiffness of the in vivo environment. 

 

> Ready to use

Plate your cells directly

 

> A wide range of stiffness available

From very soft (1 kPa) to very hard (200 kPa)

 

> A wide range of 3D designs 

Grooves, square wells, round wells, etc.

> 4DCELL GEL COATED COVERSLIPS FEATURES:

Coated with fibronectin, thus ready to be used 

Compatible with high-resolution optical microscopy systems

Provided in individual plastic bags in a water based solution that keeps the properties of the gels 

Standard shapes available: grooves and round wells

Standard size: 24 mm round coverslips (approx. thickness 170 μm)

Compatible with perfusion chambers

 

A quick and straightforward solution to study confinement of non migrating cells, since cells are directly plated on the top of 3D features. 

 

> 4DCELL GEL COATED COVERSLIPS STANDARD CATALOG:

Table Coverslips Coated with Microstructured Polyacrylamide Gels- Grooves
Table Coverslips Coated with Microstructured Polyacrylamide Gels - Round Wells

> TYPICAL ARRANGEMENT OF THE GROOVES AND ROUND WELLS IN A STANDARD 24 MM ROUND COVERSLIP:

In each coverslip there are eight areas with features of different widths (grooves) or diameters (round wells): from 10 μm to 100 μm

The depth of the structures is 10 μm

The scheme bellow depicts the particular case of the round wells, but the distribution is similar in the case of the grooves

> ADDITIONAL INFORMATION:

The size of the coverslips can be modified on demand

The same type of gels can be adapted to Petri Dishes or multiwell plates

Other shapes as triangular wells, square wells or rectangular wells can be fabricated on demand

Other depths can be fabricated on demand 

 

If this device does not meet your needs, get in touch with us! We can personalize it for you.

> CANCER

  • Cell shape standardization

 

> ORGAN PHYSIOLOGY

  • Migration of somatic and cancer cells
  • Cell shape standardization
  • Cardiomyocyte beating properties
  • Neural network
  • Detection of bile canaliculi in hepatocytes
  • Tissue geometry mimicry

 

> RARE DISEASES

  • Cell nucleus integrity
  • Nuclear plasticity
  • Neural network

 

> FUNDAMENTAL RESEARCH

  • Cell shape standardization
  • Standardized connectivity between neurons

 

> BIOMOLECULAR IN VITRO MODELS

  • Spatial autoorganization of macromolecules (at the microscale)