Mesenchymal stem cells assay

4DCELL DEVICE

Coverslips coated with microstructured gels

READ-OUTS

Observation of MSCs fate

STANDARD CULTURE LIMITATION

Mechanical and biophysical mechanisms regulate mesenchymal stem cells (MSCs) fate decision. However, these mechanisms are not well defined.

MSCs ASSAY BENEFITS

Control of MSCs differentiation is needed for many therapies such as regenerative ones [1].

Using 4Dcell gels, with tunable stiffness, cell fate can be chosen depending on the stiffness. In addition, by using 4Dcell microstructured gels and depending on the size area, cells tend to differentiate into adipocytes or osteoblasts.

EXAMPLE

MSCs differentiation depending of the stiffness [2]

By modifying the geometry and matrix elasticity of the underlying patterns, cells were able to choose lineage commitment based on the physical cues presented.

MSCs differentiation depending of the geometry [3]

By modifying the geometry and matrix elasticity of the underlying patterns, cells were able to choose lineage commitment based on the physical cues presented.

REFERENCES

[1] Piroli, M. E. et al. (2018). Annals of biomedical engineering, 46(6), 888-898.

[2] Peng, T., et al. (2017). BMC systems biology, 11(1), 55.

[3] Harris, G. M., et al.  (2014). Advanced functional materials, 24(16), 2396-2403.