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SmartSPHERO®

24 or 96 well plates

Spheroids zoom1 x19.jpg

4Dcell offers 6, 12 and 24 well plates containing hydrogel-based microwell structures specially designed for the spontaneous formation of uniform spheroids. The anti-adhesive properties of the hydrogel facilitates rapid self-assembly of cell aggregates into compact spheroids.

The APT (Anchoring Point Technology) developped by 4Dcell anchors the spheroids in place. This prevents the loss of spheroids during typical manipulations (medium exchange, drug addition, etc), and makes the location of each spheroid predictable. As the hydrogel is optically transparent it is fully compatible with conventional microscopy.

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  • Ready to use

Plate your cells directly on the microstructured substrates 

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  • Maintain +95% of spheroids, uniform size and Shape 

Keep your spheroids alive, with <6% variation of the spheroid’ size.

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  • No spheroids detachment during medium change over weeks 

The patented anchoring point technology keeps the spheroids in each microwell during medium change

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  • Predictable location and easy to image

Cell aggregates are located in predictable positions enabling automated image acquisitio

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Features & Benefits

4Dcell 24 or 96 well plates with microwells for spheroids' assembly

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  • 24 and 96 well plates with glass bottom ( #1.5, 0.16 – 0.19 mm)

  • Each macrowell has for ​24 well plates:

    • 19 or 91 individual microwells where spheroids are assembled

  • Each macrowell has for ​96 well plates:

    • 19 individual microwells where spheroids are assembled

  • The microstructured substrates are made of biocompatible polyethylene glycol gel

  • Optionally the microwells can be coated with an ECM matrix to increase the adherence of the spheroid to the contact (attachment) point

  • The gels are provided in a water based solution that keeps their mechanical and optical properties

Mesenchymal Stem cells (MSCs) seeded in a 3D structured hydrogel designed for controlled spheroid formation (4Dcell SmartSphero plates).Seeding density: 3000 cells/microwell. The video shows the initial stages of spheroid formation, including cell aggregation and spheroid compaction, followed by spheroid growth in the days following compaction due to cell proliferation.​​​

APT® - Anchoring Point Technology

Example of loss of spheroids after manipulation (with and without anchoring point)

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Game changing
Innovation

Structure and dimensions of the 96 well plate and macrowell

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Structure and dimensions of the microstructured gel with 19 microwells/well (in a 96 well plate)

SCHEME-ONE-WELL-OF-A-96-WP-19S.png
SCHEME-ONE-WELL-OF-A-96-WP-19S-TRANSVERSAL-CUT.png

Structure and dimensions of the 24 well plate and macrowell

SCHEME-24-WP-19S.png
SCHEME-ONE-WELL-OF-A-24-WP-19S-CROSS-SECTION.png

Structure and dimensions of the 24 well plate and 19 microwells/well

SCHEME-ONE-WELL-OF-A-96-WP-19S-TRANSVERSAL-CUT.png
SCHEME-ONE-WELL-OF-A-96-WP-19S.png

Structure and dimensions of the 24 well plate and macrowell

SCHEME-24-WP-19S.png
SCHEME-ONE-WELL-OF-A-24-WP-19S-CROSS-SECTION.png

Structure and dimensions of the 24 well plate and 91 microwells/well

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Spheroids – Mesenchymal stem cells (MSCs) labeled with GFP after 72 hours.

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