SmartHeart®
Circular contractile cardiac model designed to select
the right molecule in cardiotoxicity and efficacy
HUMAN HEART TISSUES
hiPSC-CMs and cardiac fibroblast
EASY TO USE +++
Plate your cells, let them beat, measure
HIGH THROUGHPUT
96-well plate
7200 CELLS:TISSUE
3:1 ration of cardiomyocytes to fibroblasts
HIGH RESOLUTION IMAGING
Glass bottom plate
IN SITU CHARACTERIZATION
Multiple key readouts in a single assay
SMARTHEART CAPITALIZE ON THE HIGHEST STANDARDS
A human-like cardiac model engineered to reach a high level of maturation
SmartHeart® tissues offer enhanced morphological, structural, molecular, and functional maturity, increasing the predictability of cardiotoxicity studies and clearly surpassing the limitations of traditional 2D approaches.
3D reconstruction of a ring. Vimentin (green), Troponin T (red) and DAPI (blue)
MORPHOLOGY
The ring-shaped geometry allows for a more physiologicallyaccurate distribution of forces within the tissue. It facilitatesthe visualization of re-entrant waves, which are responsiblefor most clinical arrhythmias.
Immunostained contractile fibers at 63X magnification. Troponin T (red) and DAPI (blue) – Seguret & al.
STRUCTURE
The SmartHeart® platform provides structurally mature tissues featuring well-aligned contractile fibers and sarcomeres.
MOLECULAR EXPRESSION
RT-qPCR data highlight the upregulation of key genes for cardiac function compared to 2D models.
3-in-1 3D cardiac assay replicating key in vivo mechanisms
The SmartHeart is an integrated solution to measure contractility (1), action potential (2), and calcium handling (3).This streamlined approach minimizes variability, delivering unmatched insights into in vivo toxic mechanisms
CONTRACTILITY (1)
Contractility Metrics
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Frequency
Contraction stress
Contraction speed
Relaxation speed
Drug cardiac tropism​
Chronotropic
Inotropic
Chronotropic
Lusitropic
ELECTROPHYSIOLOGY (2)
Action potential Metrics
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Frequency
Interpeak duration STD Rising time, Upstroke velocity
APD10-90 Triangulation, Plateau phase
Electrophysiology features
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Rhythm
Regularity Depolarization
Impacted channels Proarrhythmia
CALCIUM TRANSIENTS (3)
CaT Metrics
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Frequency
Interpeak duration STD Amplitude
Area Under curve ‘Rise and decay’ duration and speeds
CD10,50,90
Comprehensive understanding of calcium dynamics in tissues
SmartHeart® quantifies drug-induced contractile changes
CiPA Panel High-fidelity cardiac safety assessments
SmartHeart® reliably replicates the electrophysiological (via optical mapping assessment) responses to CiPA panel drugs, ensuring high-fidelity cardiac safety assessments.
HIGH TDP RISK
LOW TDP RISK
SMARTX 2.0 AI DRIVEN PRECISION FOR YOUR EXPERIMENTS
Bright field videos (e.g. 500x500 pixels, min 35 fps and 10 s length) of the contracting rings can be analyzed using 4Dcell’s SmartExplorer software, SmartX 2.0.
The algorithm uses machine learning to detect and track the area of the central pilar, from which it determines several parameters: contraction amplitude, contraction strain, contraction and relaxation speed, contraction stress, etc.
SmartX is an AI-powered image analysis platform engineered to evolve with every experiment you run.
At its core lies a deep learning module that continually “learns” from each dataset, refining its ability to detect and track beating rings with ever-greater accuracy and speed. Whether you’re measuring contraction strength, frequency, or spatial consistency, SmartX adapts to your unique imaging conditions and cell patterns, automatically adjusting its segmentation models to reduce false positives and enhance signal fidelity.
The result is a self-optimizing workflow that delivers robust, reproducible metrics experiment after experiment, freeing you to focus on biological insights rather than manual analysis.