PRECISION 3D CELL CULTURE PLATFORM
FOR CONTRACTILE TISSUES
Next-Generation 3D Cell Culture Plates
Designed for Cardiac & Skeletal Muscle Research
Easy to use
High Throughput
Low amount of cells
High resolution imaging
In situ characterization
Long term cell culture
TECHNICAL SPECIFICATIONS
CONTRACTILE ASSAY
CONTRACTILITY® IN ACTION
Our multidisciplinary team of experts developed SmartContractility®, a robust bioengineered platform for easy and efficient contractile tissue culture.​
With in situ characterization capabilities and a glass-bottom plate, our platform ensures high-resolution imaging, allowing detailed analysis and compatibility with industrial automation for faster results.​
Designed specifically for heart and skeletal muscle tissues, our system:
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Minimal Cell Requirements: Achieve robust results with significantly fewer cells than traditional methods
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Rapid Tissue Formation: Generate functional tissues in days, not weeks
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High Reproducibility: Standardized protocols ensure consistent results across experiments
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Automation Compatible: Seamlessly integrates with SLAS/ANSI standard platforms
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Real-time Analysis: Monitor contractility parameters throughout the entire culture period
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Plate formats: 6, 12, 24, 96-well options
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Compatible cell types: Cardiomyocytes, skeletal muscle cells, iPSC-derived muscle cells
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Measurement parameters: Contractility force range, temporal resolution, contraction amplitude, strain, speed, stress
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Video requirements: Bright-field, ≥35 fps, 500x500 pixels, 10 s length minimum
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Software requirements: SmartX® 2.0 (PC with GPU recommended)
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Drug Discovery & Development: Accelerate pharmaceutical research with physiologically relevant cardiac and skeletal muscle models.
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SmartContractility® supports high-throughput drug screening, toxicity assessment, and mechanism-of-action studies.
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Disease Modeling: Create accurate models for cardiovascular and muscular disorders.
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Study pathophysiology, test therapeutic interventions, and validate biomarkers with standardized, reproducible measurements.
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Academic Research: Advance fundamental understanding of muscle biology, development, and regeneration.
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Our platform supports long-term studies with consistent, publication-quality data.
PLATE YOUR CELLS. LET THEM BEAT. MEASURE WHAT MATTERS
Proprietary well design
Cells are seeded on substrates coated with a 3D structured hydrogel molded into conical-shaped microwells with a central pillar. As cells settle on these microstructures, they self-organize into circular tissues encircling the pillar.
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The deformation of the pillar, provides measurements of contractility force, beating rate, and amplitude, etc.
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The engineered shape and contact area of the pillar help secure the tissues in place.
SmartContractility® Technology applied to cardiac models
AI Analizer - SmartX® 2.0
Bright field videos (e.g. 500x500 pixels, min 35 fps and 10 s length) of the contracting rings can be analyzed using SmartX 2.0.
The algorithm uses machine learning to detect and track the area of the central pilar, from which it determines several parameters: contraction amplitude, contraction strain, contraction and relaxation speed, contraction stress, etc.
Automation driven
4Dcell's R&D team designed the SmartContractility solution to be compatible with current industrial automated platforms, adhering to SLAS/ANSI standards.
We engineered commercial plates that you already use in your routine automated experiments. This was the first step in our development journey to make your lab work significantly easier.
