5000+ ORGANOIDS

CONTROL THE 2D SHAPE
STATIC OR DYNAMIC MICROPATTERNING

PHYSIOLOGICAL ASSAY AT A 2D LEVEL

2D cell culture models remain a cornerstone in biological research and drug discovery, offering a controlled and reproducible environment for studying cellular behavior.

While traditional monolayer cultures provide valuable insights, integrating biophysical cues into 2D assays enhances physiological relevance.

Our R&D experts leverage microfabrication and biomaterial engineering to refine 2D physiological assays, ensuring cells experience spatial constraints and microenvironmental cues that better mimic in vivo conditions.

We have developed a full range of formats to seamlessly integrate into your research projects, from coverslips and petri dishes to high-throughput 384-well plates. Whatever your experimental setup, our solutions are designed to provide flexibility and adaptability while maintaining reproducibility and precision.

The SmartPattern Technology enables the study of contractility, migration, differentiation, and mechanotransduction in a more physiologically relevant manner, bridging the gap between traditional 2D cultures and more complex 3D models.

Easy to use

High Throughput

High resolution imaging

Long term cell culture

PLATE YOUR CELLS. LET THEM ADHERE. MEASURE.

STATIC MICROPATTERN

Cells are cultured in a substrate patterned with adherent micrometer-sized domains of predefined geometric features. When cultured on this, cells adhere specifically to the patterned area, acquiring for example, the geometry of the pattern.

Cell culture supports receive a covalently bounded surface chemistry treatment with an oxidation resistance anti-adhesive polymer. Less sensitive to oxidation than a classical polymer with the same type of covalent bonding to the glass slide. After plated you can keep your cells in the patterns up to 2 weeks. Non used substrates can be stored up to 6 months.

Different pattern shapes and dimensions (lines, squares, triangles, rectangles, grids, etc.)
Customized shapes and dimensions
Adaptable to any cell culture substrate (from Petri dishes to 384 well plates) – from single cell to high throughput and high content screening analysis
Compatible with high-resolution optical microscopy systems

SmartPattern® catalog
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DYNAMIC MICROPATTERN

Dynamic SmartPattern combines 4Dcell’s micropatterning technology and click chemistry to induce cell migration of cells from pre-defined geometries.

Cells are seeded on a micropatterned substrate and specifically adhere to cell-adhesive regions with very high resolution. The regions around the cells are non adhesive at first but can be ‘switched on’ at any time to release the cells from their patterns.

As with standard micropatterning, it is possible to pattern the cells into customized shapes and dimensions.

Anti-adhesive polymer

Covalent and electrostatic bonding to the glass slide, stable for up to 3 months

The polymer can be rendered adhesive by a click chemistry reaction

Time lapse of Mesenchymal Stem Cells (MSCs) migration on 4Dcell dynamic micropatterns

The cells are initially patterned in 500 µm disks. At t0, the cells are released from their patterns and migrate while maintaining a circular shape.

MICROPATTERN APPLICATIONS

Cell shape control and standardization, characterization of organelle positioning, cell maturation and differentiation, restriction of the migration area, cellular reprogramming, protein micropatterning, etc.