Cells are cultured in a substrate patterned with adherent micrometer-sized domains of predefined geometric features. When cultured on this, cell adhere specifically to the patterned area, acquiring for example, the geometry of the pattern.
Different covalently bounded surface chemistry (pattern life time, cell nature):
Standard anti-adhesive polymer PEG
Oxidation resistance anti-adhesive polymer PMOXA
Long term cell culture anti-adhesive polymer PNIPAM
Different pattern shapes and dimensions (lines, squares, triangles, grids, etc.)
Adaptable to any cell culture substrate (from Petri dishes to 384 well plates)
Compatible with high-resolution optical microscopy systems
Cell shape control and standardization, characterization of organnel positionning, cell maturation and differentiation, restriction of the migration area, etc.
HeLa cells in lines of 100 to 10 µm width
Explore examples of applications