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New technologies to culture spheroids 

Spheroids are 3D aggregates of cells that spontaneously form in culture. These in vitro models rely on cell-cell interactions rather than interactions between the cell and the tissue culture substrate, and are phetoypically and biochemically more similar to cells in vivo than classical tissue cultures. In this webinar we present 4Dcell’s new technology based on micro-structured based substrate, with microwells, where spheroids can self-assemble, with homogenous and reproducible size and shape. These microwells contain an anchoring point at the bottom, which enables the immobilization of the spheroids thus precluding their loss during manipulation.

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New technologies to study cell migration 

Cell migration is an essential event involved in many physiological and pathological processes. In this webinar we present various technologies that can be employed to study cell migration in conditions that optimally mimic the in vivo microenvironment. The advantages of these tools (e.g., cell confiner, microchannels, micropatterns) over conventional cell culture methods are discussed, and practical examples are shown. 

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New technologies to study cell differentiation

Cells undergo differentiation as they mature and specialize in different functions. The cellular differentiation is a vital process in living organisms, regulated by mechanical and chemical stimuli. The use of appropriate lab tools to study cell differentiation in vitro is of crucial importance. In this webinar, we present several technologies (e.g., micropatterns, cell confiner, 3D hydrogels), that can be employed to improve and ensure proper cell differentiation. 

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Dynamic micropatterns as a tool to study wound healing 

Wound healing is a vital and complex process in physiological and pathological states. It is divided into four main stages: inflammation, growth, re-epithelialization, and remodeling. Each of these phases are mediated by several cell types, which coordinate the molecular mechanisms that ultimately lead to wound closure. In this webinar, we will discuss various technologies and scientific applications that can help studying the wound healing process (e.g. cell proliferation, migration, and contractility).

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