Cells are cultured in a soft 3D environment, either flat or microstructured, to mimic in vivo conditions.
Stiffness of the substrate can be chosen from very hard (200 kPa) to very soft (1 kPa)
Wide range of substrates topography available (flat, wells, grooves…)
Rapid to use since cells are seeded directly on the top of the features (convenient to confine non migrating cells)
Can be confined with several ECM proteins
Adaptable to any cell culture substrate (from Petri dishes to 96 well plates)
Compatible with high-resolution optical microscopy systems
Cell differentiation, Cell mechanics, Cell contractility, Cell migration, Tissue geometry mimicry, 3D cell shape control, etc.
HeLa cells on 4Dcell’s microstructured gel
- Cardiac spheroids as promising in vitro models to study the human heart microenvironment
Polonchuk, L., et al. Scientific reports, 2017 7(1), 7005
- Current and emerging modalities for detection of cardiotoxicity in cardio-oncology
Khouri, M. G., et al. Future cardiology, 2015 11(4), 471-484
- Bioinspired living structural color hydrogels
Fu, F., et al. Science Robotics, 2018 3(16), eaar8580
- Preparation of hydrogel substrates with tunable mechanical properties
Tse, J. R., & Engler, A. J. Current protocols in cell biology, 2010 47(1) 10-16
- Differentiation of liver progenitor cell line to functional organotypic cultures in 3D nanofibrillar cellulose and hyaluronan-gelatin hydrogels
Malinen, M. M., et al. Biomaterials, 2014 35(19), 5110-5121.