Gels

Control the 3D structure and mechanics of your cells

TECHNOLOGY

Cells are cultured in a 3D environment (different stiffnesses, either flat or microstructured, to mimic in vivo conditions.

 

> Stiffness of the substrate can be chosen from very soft (1 kPa) to very hard (50 kPa)

> Wide range of substrates topography available (flat, round wells, square wells, grooves, etc.)

> The gel based substrates are ready to be used in your cell culture experiments 

> Easy and straightforward to use, since cells are seeded directly on top of the features (convenient to confine non migrating cells)

> Pre-coated with ECM matrix (e.g. fibronectin) 

> Adaptable to any cell culture substrate (coverslips, Petri dishes, multiwell plates)

> The optically transparency of the gels makes these substrates compatible with high-resolution optical microscopy systems

> Contact us if you want a customized design

APPLICATIONS

Cell differentiation, Cell mechanics, Cell contractility, Cell migration, Tissue geometry mimicry, 3D cell shape control, etc.

HeLa cells on 4Dcell’s microstructured gel

PUBLICATIONS

  • Adherens junction engagement regulates functional patterning of the cardiac pacemaker cell lineage
    Thomas et al., 2021, Developmental Cell 56, 1498–1511
  • Cardiac spheroids as promising in vitro models to study the human heart microenvironment
    Polonchuk, L., et al. Scientific reports, 2017 7(1), 7005
  • Current and emerging modalities for detection of cardiotoxicity in cardio-oncology
    Khouri, M. G., et al. Future cardiology2015 11(4), 471-484
  • Bioinspired living structural color hydrogels
    Fu, F., et al. Science Robotics, 2018 3(16), eaar8580
  • Preparation of hydrogel substrates with tunable mechanical properties
    Tse, J. R., & Engler, A. J. Current protocols in cell biology, 2010 47(1) 10-16
  • Differentiation of liver progenitor cell line to functional organotypic cultures in 3D nanofibrillar cellulose and hyaluronan-gelatin hydrogels
    Malinen, M. M., et al. Biomaterials2014 35(19), 5110-5121.

PRODUCTS