Hydrogels

3D cell environment control using microstructured substates

TECHNOLOGY

STRUCTURED GELS

Cells are cultured in a 3D environment (different stiffnesses, either flat or microstructured, to mimic in vivo conditions).

> Stiffness of the substrate can be chosen from very soft (1 kPa) to very hard (50 kPa)

> Wide range of substrates topography available (flat, round wells, square wells, grooves, etc.)

> The gel based substrates are ready to be used in your cell culture experiments 

> Easy and straightforward to use, since cells are seeded directly on top of the features (convenient to confine non migrating cells)

> Pre-coated with ECM matrix (e.g. fibronectin) 

> Adaptable to any cell culture substrate (coverslips, Petri dishes, multiwell plates)

> The optically transparency of the gels makes these substrates compatible with high-resolution optical microscopy systems

> Contact us if you want a customized design

MICROWELLS – ASSEMBLY OF SPHEROIDS

Cells are cultured on a substrate with microwells enabling the spontaneous formation of spheroids 

> The size of the spheroids depends only on the amount of cells

> The microwells have a contact point enabling the spheroid to stay anchored during manipulation 

> In one well you can have many spheroids (up to 91) with reproducible sizes 

> The substrates are ready to be used in your cell culture experiments 

> Easy and straightforward to use, since cells are seeded directly on top of the features and spontaneously form aggregates of cells in the format of spheroids 

> Adaptable to any cell culture substrate (coverslips, Petri dishes, multiwell plates)

> The optically transparency of the gels makes these substrates compatible with high-resolution optical microscopy systems

> Get in touch with us if the microwells design do suit your precise needs 

APPLICATIONS

STRUCTURED GELS 

Cell differentiation, Cell mechanics, Cell contractility, Cell migration, Tissue geometry mimicry, 3D cell shape control, etc.

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HeLa cells seeded on microstructured gels

image (5)

Mouse embryonic fibroblasts seeded on grooves 

MICROWELLS – ASSEMBLY OF SPHEROIDS

Formation of spheroids, tissue structure, signaling pathways, immune activation, etc. 

MSCs cells form spheroids when cultured in PEG hydrogels with microwells

FLUORESCENCEadjusted green 3

MSCs (GFP) cells form spheroids when cultured in PEG hydrogels with microwells

PUBLICATIONS

  • Adherens junction engagement regulates functional patterning of the cardiac pacemaker cell lineage
    Thomas et al., 2021, Developmental Cell 56, 1498–1511
  • Cardiac spheroids as promising in vitro models to study the human heart microenvironment
    Polonchuk, L., et al. Scientific reports, 2017 7(1), 7005
  • Current and emerging modalities for detection of cardiotoxicity in cardio-oncology
    Khouri, M. G., et al. Future cardiology2015 11(4), 471-484
  • Bioinspired living structural color hydrogels
    Fu, F., et al. Science Robotics, 2018 3(16), eaar8580
  • Preparation of hydrogel substrates with tunable mechanical properties
    Tse, J. R., & Engler, A. J. Current protocols in cell biology, 2010 47(1) 10-16
  • Differentiation of liver progenitor cell line to functional organotypic cultures in 3D nanofibrillar cellulose and hyaluronan-gelatin hydrogels
    Malinen, M. M., et al. Biomaterials2014 35(19), 5110-5121.

PRODUCTS