SMART CONTROL OF THE CELL

 

MICROENVIRONMENT

 

BIOENGINEERED IN VITRO ASSAYS

 

OUR PRODUCTS

2D and “2.5D” bioengineered cell culture consumables to control the cell microenvironment

4Dcell is a pioneer in the development and commercialization of microfabricated systems dedicated to the control of the cell microenvironment. Our team brings all its expertise to help you in your experiments, allowing you to create the ultimate cell microenvironment.

Micropatterning process

> MICROPATTERNS

 

Adhesive surfaces allowing a better control of the spatial organization of cells. Better control cell environment and thus the cytoskeleton architecture, as well as polarity, migration, division, growth, and differentiation of cells.

Microchannels migration

> MICROCHANNELS

 

Microfluidics devices with a specific geometry compatible with high-resolution microscopy. Study non-adherent cell migration under controlled conditions mimicking their physiological environment.

Cell confinement process

> CELL CONFINER

 

Homogenous confinement of cells in culture by ensuring confinement of cells between two parallel planes (covered with micropillars). Cells move in 2 dimensions and the height of the cells is precisely imposed (geometric confinement).

Gel cell culture principle

> GELS

 

Polymer whose stiffness can be tuned to replicate the exact environment your cells need to grow. This substrate allows maturation, differentiation, and polarization of the cells in a 3D environment.

HOW CAN 4DCELL BRING BIOENGINEERING TO OPTIMIZE YOUR IN VITRO ASSAYS?

EXAMPLES OF APPLICATIONS

oncology

> CANCER RESEARCH

Cell mechanics or cell organization are key parameters for cancer development and metastasis.

4Dcell enables better control of these parameters and offer new read-outs to decipher cancer progression and invasiveness.

For instance, spindle assembly disorders of cancerous cells are easily assessed via Cell confinement technology.

immunology

> IMMUNOLOGY

Sensing and migration in the tissues is the basis of the immune system functionning.

4Dcell technologies are the best tools to quantitfy immune activities such as cell migration, cell-interaction, nucleus plasticity, among others.

For instance, 4Dcell Microchannels can quantify leukocyte extravasation ability, with read-outs such as: cell speed, persistence and ability to pass through constricted spaces.

line patterned cardiomyocytes

> ORGAN PHYSIOLOGY

The regular physiology of cells in living organs is closely linked with its spatial and mechanical organization.

4Dcell offers simple and representative models highlighting organ specific read-outs.

For instance, 4Dcell Micropatterns induce striation of cardiomyocyte leading to standardized beating properties and internal organization.

rare disease

> RARE DISEASES

Rare diseases are often linked to cell mechanics or cell organization disorders.

The 4Dcell technologies highlight alterations like these and one can measure molecular modifications more easily than with other models.

For instance, cell nucleus healing in laminopathy is evaluated using Cell confinement by the observation of lamina reconstruction as a read-out.

LATEST UPDATES

> CELL MIGRATION ASSAY 

Analysis of mechanisms occurring during migration

Micropatterning methods allow the reconstitution of tissue-like conditions for in vitro cell culture to mimic migration under high geometrical constraints that take place in vivo. With this 4Dcell assay, read more …

TRAFFICKING ASSAY

Cell confinement for cytoskeletal organization

Using 4Dcell’s micropatterns, the components of the cytoskeleton are spatially separated, and cells do not change their shapes upon removal of the F-actin bundles. With this 4Dcell assay, read more …

> CHEMOTAXIS ASSAY

Analysis of the cell behaviors and cell migration in confined spaces

With the 4Dcell assay, you can analyze the migration of many cell types after the establishment of a chemotactic gradient generated by positive pressure between inlet and outlet wells,  read more …

> CELL MIGRATION IN CANCER

Analysis of cell mechanisms during metastasis

Using 4Dcell microchannels, natural constraints can be reproduced to mimic the trajectory of cancer cells spreading away from the primary tumor. Read more …

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