BIOENGINEERED IN VITRO ASSAYS 

for DRUG DISCOVERY, TOXICOLOGY & ACADEMIC RESEARCH

OUR PRODUCTS

3D, “2.5D” and 2D bioengineered cell culture consumables to control the cell microenvironment, and create innovative and perfectly adapted cell models.

4Dcell is a pioneer in the development and commercialization of microfabricated systems dedicated to the control of the cell microenvironment. With hundreds of customers around the globe, we answered many specific requests about all kinds of cell types and applications. With this strong expertise, 4Dcell solutions allow you to develop reproductible and robust cell models to design the perfect assay

Picto contractile assay
Contractile assay

FIRST HCS CONTRACTILE ASSAY

Self assembly of cell aggregates with a ring shape that  that facilitate contractility force measurements

Spheroids process
Spheroids solution

CUTTING EDGE TECHNOLOGY

Hydrogel based microwells to form functional and reproducible spheroids. An anchoring point precludes them from being lost during medium exchange.

Micropatterning process

Static or switchable adhesive patterns allowing a geometric control of the cells. 

Cell confinement process

Homogenous confinement of cells between two parallel planes to a defined micrometer height with sub micrometer resolution. 

Microchannels migration
Picto microchannels

PDMS based microchannels for cell migration and nuclear deformation

Picto micropillars
Micropillars

Substrates with micropillars to assess single cell contractility

Gel cell culture principle
Hydrogels

Controlled stiffness of the cell substrate

APPLICATIONS

A widespread range of applications for 4Dcell 2D and ”2.5D” tools, covering the hot topics of the moment in cell biology. 4Dcell technologies can be applied to several cell types and enable the acquisition of distinct readouts. 

BY CELL TYPE

IMMUNE CELLS

Immune cells

Improve tracking and imaging of immune cells, imitate phagocytosis and improve the phenotypes of your cells
CARDIOMYOCYTES

Cardiomyocytes

Improve differentiation of neonatal and iPSC-derived cardiomyocytes, quantify contractility
MUSCLE CELLS

Muscle cells

Improve differentiation of myoblasts to myotubes, quantify contractility and imitate the neuromuscular junction
HEPATOCYTES

Hepatocytes

Improve cell-cell interaction and imaging
NEURONS

Neurons

Improve neuronal network formation and imitate the neuromuscular junction
CANCER CELLS

Cancer cells

Observe cancer cell migration in conditions that imitate metastasis, and quantify aggressiveness and contractility
FIBROBLAST

Fibroblasts

Trigger migration using click chemistry, quantify cell contractility
STEM CELLS

Stem cells

Manipulate stem cell differentiation using cell patterning and substrate stiffness
ENDOTHELIAL CELLS

Other cell types

Assays for bone cells, adipocytes, endothelial cells, ciliated cells, and other general applications

BY READOUT

Cell migration

Cell migration

Confine migration in 2D or 3D to facilitate tracking, mimic migration through tissues, and study chemotaxis
Cell organization & phenotype

Cell organization & phenotype

Restrict cell adhesion in 2D to promote differentiation or improve reproducibility of your assays
Cell differentiation

Cell differentiation

Drive cell differentiation by controlling substrate stiffness and confining cells in 2D and 3D
Cell-cell interaction

Cell-cell interaction

Confine your cells in 2D to better image interaction between one or more cell type
Cell contractility

Cell contractility

Quantify the contractility of cells on gels or micropillars
Cell membrane events

Cell membrane events

Mimic phagocytosis, Observe endo- or exocytosis, and generate exosomes
Mitosis and nucleus related events

Mitosis & nucleus related events

Quantify nucleus fragility and the ability of the cell to perform mitosis under stress
Co-cultures

Co-cultures

Analysis of the cell-cell contact by click chemistry technology
Cell volume measurements

Cell volume

Accurately measure the volume of your cells
Spheroïds and readouts

Other readouts

Spheroid formation, blood vessel formation, neuromuscular junction and more

HOW CAN 4DCELL BRING BIOENGINEERING TO OPTIMIZE YOUR IN VITRO ASSAYS?

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