SMART CONTROL OF THE CELL

 

MICROENVIRONMENT

 

BIOENGINEERED IN VITRO ASSAYS

 

OUR PRODUCTS

2D and “2.5D” bioengineered cell culture consumables to control the cell microenvironment

4Dcell is a pioneer in the development and commercialization of microfabricated systems dedicated to the control of the cell microenvironment. Our team brings all its expertise to help you in your experiments, allowing you to create the ultimate cell microenvironment.

Cell confinement process

> CELL CONFINER

 

Homogenous confinement of cells in culture by ensuring confinement of cells between two parallel planes (covered with micropillars). Cells move in 2 dimensions and the height of the cells is precisely imposed (geometric confinement).

Micropatterning process

> MICROPATTERNS

 

Adhesive surfaces allowing a better control of the spatial organization of cells. Better control cell environment and thus the cytoskeleton architecture, as well as polarity, migration, division, growth, and differentiation of cells.

Microchannels migration

> MICROCHANNELS

 

Microfluidics devices with a specific geometry compatible with high-resolution microscopy. Study non-adherent cell migration under controlled conditions mimicking their physiological environment.

Gel cell culture principle

> GELS

 

Polymer whose stiffness can be tuned to replicate the exact environment your cells need to grow. This substrate allows maturation, differentiation, and polarization of the cells in a 3D environment.

HOW CAN 4DCELL BRING BIOENGINEERING TO OPTIMIZE YOUR IN VITRO ASSAYS?

EXAMPLES OF APPLICATIONS

oncology

> CANCER RESEARCH

Cell mechanics or cell organization are key parameters for cancer development and metastasis.

4Dcell enables better control of these parameters and offer new read-outs to decipher cancer progression and invasiveness.

For instance, spindle assembly disorders of cancerous cells are easily assessed via Cell confinement technology.

immunology

> IMMUNOLOGY

Sensing and migration in the tissues is the basis of the immune system functionning.

4Dcell technologies are the best tools to quantitfy immune activities such as cell migration, cell-interaction, nucleus plasticity, among others.

For instance, 4Dcell Microchannels can quantify leukocyte extravasation ability, with read-outs such as: cell speed, persistence and ability to pass through constricted spaces.

line patterned cardiomyocytes

> ORGAN PHYSIOLOGY

The regular physiology of cells in living organs is closely linked with its spatial and mechanical organization.

4Dcell offers simple and representative models highlighting organ specific read-outs.

For instance, 4Dcell Micropatterns induce striation of cardiomyocyte leading to standardized beating properties and internal organization.

rare disease

> RARE DISEASES

Rare diseases are often linked to cell mechanics or cell organization disorders.

The 4Dcell technologies highlight alterations like these and one can measure molecular modifications more easily than with other models.

For instance, cell nucleus healing in laminopathy is evaluated using Cell confinement by the observation of lamina reconstruction as a read-out.

LATEST UPDATES

> 4DCELL AT BIOFIT 

Very interesting opportunities

With the highest attending rate of academics, TTOs and research institutions, BioFIT is the place where academia-industry collaborations get started. Very interesting opportunities in perspective on 4Dcell “SmartMicroOrgans” innovative in vitro models.

> LIVE CELL IMAGING

Observing cells by time-lapse microscopy

Using 4Dcell micropatterns, cells are cultured on substrate patterned with adherent domains of predefined geometric features in a standard glass-bottom Petri dish that enables to observe them by time-lapse microscopy.  Read more …

> ORGANELLES POSITIONING 

Precise statistics on organelle positioning in the cell

Using 4Dcell micropatterns, cells  acquire a standard and reproducible shape. This enables the acquisition of high-resolution images, from which precise statistical models on the exact positioning of the organelles can be derived.  Read more …

> CELL NUCLEUS SQUEEZING 

Precise control of nuclear membrane rupture

In standard cultures, cells are distributed in 2D monolayers causing them to acquire random shapes. When cells are cultured in microchannels, the cells behavior can be tracked, and it enables the acquisition of high-resolution images. Read more …

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