Cancer invasiveness assays

Quantification of migration behaviors and migration transitions

4DCELL DEVICE

Cell Confiner

 

READ-OUTS

Cell adhesion, quantification of cell migration speed, cell migration phenotype, cell migration transition

 

STANDARD CULTURE LIMITATION

Most cells on a flat surface show a “mesenchymal”-like migration behavior which is not representative from their in-vivo behavior.

 

CANCER INVASIVENESS OF CELLS

In an environment controlled in adhesiveness and confinement level, migration transitions (e.g. mesenchymal to amoeboid – MAT) can be trigger, observed and characterized (quantified).

 

EXAMPLE

Slow mesenchymal cells can switch to fast amoeboid-like migration under conditions of low adhesion and strong confinement [1].

Confined cells

REFERENCES

[1] Liu, Y.-J; et al. (2015). Cells. Cell, 160