Cancer invasiveness assays

Quantification of migration behaviors and migration transitions

4DCELL DEVICE

Cell Confiner

 

READ-OUTS

Cell adhesion, quantification of cell migration speed, cell migration phenotype, cell migration transition

 

STANDARD CULTURE LIMITATION

Most cells on a flat surface show a “mesenchymal”-like migration behavior which is not representative of their in vivo behavior.

 

CANCER INVASIVENESS OF CELLS

In an environment controlled in adhesiveness and confinement level, migration transitions (e.g. mesenchymal to amoeboid – MAT) can be triggered, observed and characterized (quantified).

 

EXAMPLE

Slow mesenchymal cells can switch to fast amoeboid-like migration under conditions of low adhesion and strong confinement [1].

Confined cells

REFERENCES

[1] Liu, Y.-J; et al. (2015). Cells. Cell, 160