> DYNAMIC CONFINER

A step towards mimicking the natural cell environment

Cell Confiner - Pack microscope

The dynamic cell confiner is a device that allows confining cells between two parallel surfaces with sub-micrometer precision. The space between the two surfaces is controlled by micro PDMS pillars. The micro pillars are fabricated in a glass slide, which is attached to a PDMS piston (suction cup). The piston is controlled with a vacuum pump, and the height of the confinement is thus controlled as well.

The device can be used in a petri dish, in a glass substrate, etc.

 

> Control the confinement speed

Accurate control of the confinement speed through the vacuum pump

 

> Reversible confinement: retrieve your cells after confinement

Allows molecular analysis thanks to the non-destructive method for the cells

 

> Compatible with your own experiments

The confiner is a small size device that is directly placed on the top of your cell culture

> THE DYNAMIC CELL CONFINER KIT IS COMPOSED BY:

 

1 x Elveflow Vacuum + Pressure Generator and 1 x Elveflow AF1 DUAL – Vacuum + Pressure Controller

10 x Confinement Slides – 10 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement.
Available confinement heights – from 1, 2, 3, … up to 20 um (choose up to 3 heights to integrate your kit)

3 x 4Dcell Dynamic Confiner™ (PDMS Suction Cup)

1 x security tank (to preserve the vacuum pump)

1 x Kit of Tubes and Connectors

 

> IF YOU WANT TO MAKE YOUR SLIDES ADHESIVE TO YOUR CELLS OR NOT (OPTIONAL):

 

Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution

Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides

> CANCER

  • Migration of metastatic cells
  • Cell contractility in mestastasis
  • DNA DSB repair (mechanically induced)
  • Genomic instability (cell division)
  • Separated co-culture

 

> IMMUNOLOGY

  • Migration of immune cells
  • Imaging of non-adhesive cells

 

> ORGAN PHYSIOLOGY

  • Migration of cancer cells
  • Cell differenciation with stiffness control
  • Wound healing
  • Separeted co-culture
  • Cell compression response

 

> RARE DISEASES

  • Cell nucleus integrity

 

> AGING

  • Cell nucleus integrity
  • Autophagy related diseases

 

> OBSERVATION OPTIMIZATION

  • Imaging of non-adhesive cells
  • Planar imaging of organelles

 

> FUNDAMENTAL RESEARCH

  • Cell volume (cell cycle)
  • Cell stretching response

Video of HeLa cells under confinement using a 4Dcell confiner, going from initial state to extremely confined.

Video of cells dividing under confinement using a 4Dcell confiner

Example of mammalian cells with before and after confinement images with fluorescent proteins
Le Berre M, Aubertin J, Piel M., Fine control of nuclear confinement identifies a threshold deformation leading to lamina rupture and induction of specific genes. Integr Biol (Camb). 2012 Nov;4(11):1406-14.

Confinement and Low Adhesion Induce Fast Amoeboid Migration of Slow Mesenchymal Cells
Y.-J. Liu, M. Piel, Cell, et al., 2015 160(4), 659-672

 

Actin flows induce a universal coupling between cell speed and cell persistence
P. Maiuri, R. Voituriez, et al., Cell, 2015 161(2), 374–386

 

Geometric friction directs cell migration
M. Le Berre, M. Piel, et al., Physical Review Letter 2013 111, 198101

 

Mitotic rounding alters cell geometry to ensure efficient spindle assembly
O. M. Lancaster, B. Baum, et al., Developmental Cell, 2013 25(3), 270-283

 

Fine Control of Nuclear Confinement Identifies a Threshold Deformation leading to Lamina Rupture and Induction of Specific Genes
M. Le Berre, J. Aubertin, M. Piel, Integrative Biology, 2012 4 (11), 1406-1414

 

Exploring the Function of Cell Shape and Size during Mitosis
C. Cadart, H. K. Matthews, et al., Developmental Cell, 2014 29(2), 159-169

 

Methods for Two-Dimensional Cell Confinement
M. Le Berre, M. Piel, et al., 2014, Micropatterning in Cell Biology Part C, Methods in cell biology, 121, 213-29