Coculture assay

Analysis of the cell-cell contact by click chemistry technology


Micropatterns (Dynamic)



Cell-cell contact, cells interaction



In standard conditions, the simplest co-culture system that permits physical contact between cells consists of a mixed monolayer of the cell types of interest. One limitation is the metabolic differences between cell types. Moreover, the relative contribution of each cell population to any observed effects is not readily discernible in this model [1]. 



With the 4Dcell assay, your coculture is controlled in space and time. Briefly, you plate your first cell type and let them fill the available area. Then after the trigger, the second cell type can be added and will fill the newly available areaThis technique profits from the fact that the “second” cells are quick to adhere from solution, while the “first” cells are relatively slow to spread and migrate onto the newly available surface [2]. 



Organization of the different cell types [3]

U2OS cells on 200 µm disks (green) were seeded. After cells filled completely the disks, RPE1 cells (red) were added.

Then, fluorescent microscopy was used to analyze the organization of the cells. 

coculture microscopy images