Mitotic assembly assays

Quantification of mitotic spindle disorders

4DCELL DEVICE

Cell Confiner

 

READ-OUTS

Spindle assembly, chromosome segregation, flat spindle, spindle robustness, spindle dynamics, fine analysis of microtubules, centrosomes, kinetochores and interaction of other organelles .

 

STANDARD CULTURE LIMITATION

The mitotic spindle is a compact 3D structure assembling in a very short time with many redundant mechanisms which makes it tedious to analyze, especially its subtle changes.

 

MITOTIC ASSEMBLY ASSAYS

In this assay cells are observed while compressed in between two parallel surfaces, which gives them a flat shape. In this configuration, events such as spindle assembly, spindle robustness, interactions between organelles can be easily observed. In addition, the mechanical stress imposed on the spindle make it easier to destabilize allowing measurements of subtle changes in its mechanisms of assembly.

 

EXAMPLE

Effect of confinement in mitosis of HeLa cells [1].

Confinement application

REFERENCES

[1] Lancaster, O. et al.(2013). Developmental Cell, 25(3), 270–283.

[2] Cadart, C. et al.(2014). Developmental Cell, 29(2), 159–169.