SmartSphero Plates: Microwells for spheroid assembly 

Explore the potential of our anchored spheroid plates to form functional and reproducible spheroids

Photo 6 well plate_final

4Dcell offers 6, 12 and 24 well plates containing hydrogel-based microwell structures specially designed for the spontaneous formation of uniform spheroids.  The anti-adhesive properties of the hydrogel facilitates rapid self-assembly of cell aggregates into compact spheroids.

The adhesive bottom surface of each microwell anchors the spheroids in place. This prevents the loss of spheroids during typical manipulations (medium exchange, drug addition, etc), and makes the location of each spheroid predictable. As the hydrogel is optically transparent it is fully compatible with conventional microscopy.

 

> READY TO USE

Plate your cells directly on the microstructured substrates 

 

> KEEP YOUR SPHEROIDS IN PLACE 

The anchoring point helps keep the spheroids in each microwell during medium exchanges

 

> REPRODUCIBLE SIZE AND SHAPE 

The size of the spheroids depends only on the cell seeding concentration

 

> PREDICTABLE LOCATION AND EASY TO IMAGE

Cell aggregates are located in predictable positions enabling automated image acquisition

HOW TO HANDLE YOUR 6 WELL PLATES 

Spheroids protocol

FEATURES & BENEFITS

4DCELL 6 WELL PLATES WITH MICROWELLS FOR SPHEROIDS’ ASSEMBLY:

 

6, 12 and 24 well plates with glass bottom ( #1.5, 0.16 – 0.19 mm), micro-well size 14 mm or 10 mm

Each macrowell has 91 individual microwells where spheroids are assembled

The microstructured substrates are made of biocompatible polyethylene glycol gel

Optionally the microwells can be coated with an ECM matrix to increase the adherence of the spheroid to the contact (attachment) point

The gels are provided in a water based solution that keeps their mechanical and optical properties 

SCHEME OF THE PLATE AND MACROWELL:

SCHEME OF THE MICROSTRUCTURED GEL AND MICROWELL:

EXAMPLE OF LOSS OF SPHEROIDS AFTER MANIPULATION (WITH AND WITHOUT CONTACT POINTS): 

CANCER

 

Signaling pathways 

Immune activation  

Tissue Structure  

 

DRUG SCREENING 

 

REGENERATIVE MEDICINE 

 

TOXICOLOGY

Spheroids – Mesenchymal stem cells (MSCs) labeled with GFP after 72 hours.