EXPLORE CELL MECHANICS 1-WELL STATIC CONFINER – CSOW 110

1-well Cell confiner

The 1-well static confiner is a portable device that confines cells between two surfaces to a defined micrometer height with nanometer precision. The space between the two surfaces is controlled by using micro PDMS pillars. The micropillars are fabricated on a glass slide, which is attached to a PDMS piston.

The CSOW 110 is the device that controls the piston mechanically and it is compatible with glass bottom petri dishes (35 mm). The cells can be retrieved after confined, enabling further biochemical analysis. The user decides when and where to confine the cells as it is not necessary to plug it to any other device.

> USER FRIENDLY AND CONVENIENT

It is easy to assemble and is portable

> ENABLES HIGH RESOLUTION IMAGING 

It fits with glass bottom petri dishes (35 mm) and the observation area is made of optically transparent materials

> ADAPTABLE WITH YOUR MICROSCOPE

It was designed to fit in your microscope

HOW TO SET UP YOUR 1-WELL STATIC CONFINER – CSOW 110

Cell static confinement in 1 well

FEATURES & BENEFITS

THE STATIC CELL CONFINER CSOW 110 IS COMPOSED OF:

> 1 x CSOW 110

> 12 x Confinement Slides – 16 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement

Available confinement heights – from 1, 2, 3, … up to 20 µm (you can choose up to 3 heights to integrate your kit)

> 2 x Confinement Piston in PDMS

IF YOU WANT TO MAKE YOUR SLIDES ADHESIVE TO YOUR CELLS OR NOT (OPTIONAL):

Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution

Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides

If this device does not meet your needs, get in touch with us! We can personalize it for you.

VIDEO PROTOCOL TO SET UP PERFECTLY YOUR CSOW 110

ANATOMY OF THE CSOW 110

SchemeCSOW110
dimensions_3

Overall dimensions of the CSOW 110

Overview_final

The lid enables access to the petri dish, allowing cell media to be exchanged while cells are confined.

CANCER

> Migration of metastatic cells

> Cell contractility in mestastasis

> DNA DSB repair (mechanically induced)

> Genomic instability (cell division)

> Separated co-culture

IMMUNOLOGY

> Migration of immune cells

> Imaging of non-adhesive cells

ORGAN PHYSIOLOGY

> Migration of cancer cells

> Cell differenciation with stiffness control

> Wound healing

> Separeted co-culture

> Cell compression response

RARE DISEASES

> Cell nucleus integrity

AGING

> Cell nucleus integrity

> Autophagy related diseases

OBSERVATION OPTIMIZATION

> Imaging of non-adhesive cells

> Planar imaging of organelles

FUNDAMENTAL RESEARCH

> Cell volume (cell cycle)

> Cell stretching response

Video of HeLa cells under confinement using a 4Dcell confiner, going from initial state to extremely confined.

Video of cells dividing under confinement using a 4Dcell confiner

Example of mammalian cells with before and after confinement images with fluorescent proteins

> Confinement and Low Adhesion Induce Fast Amoeboid Migration of Slow Mesenchymal Cells
Y.-J. Liu, M. Piel, Cell, et al., 2015 160(4), 659-672

> Actin flows induce a universal coupling between cell speed and cell persistence
P. Maiuri, R. Voituriez, et al., Cell, 2015 161(2), 374–386

> Geometric friction directs cell migration
M. Le Berre, M. Piel, et al., Physical Review Letter 2013 111, 198101

> Mitotic rounding alters cell geometry to ensure efficient spindle assembly
O. M. Lancaster, B. Baum, et al., Developmental Cell, 2013 25(3), 270-283

> Fine Control of Nuclear Confinement Identifies a Threshold Deformation leading to Lamina Rupture and Induction of Specific Genes
M. Le Berre, J. Aubertin, M. Piel, Integrative Biology, 2012 4 (11), 1406-1414

> Exploring the Function of Cell Shape and Size during Mitosis
C. Cadart, H. K. Matthews, et al., Developmental Cell, 2014 29(2), 159-169

> Methods for Two-Dimensional Cell Confinement
M. Le Berre, M. Piel, et al., 2014, Micropatterning in Cell Biology Part C, Methods in cell biology, 121, 213-29