The static confiner is a portable device that allows confining cells within two surfaces with nanometer precision. The space between the two surfaces is controlled by using micro PDMS pillars. The micro pillars are fabricated in a glass slide, which is attached to a PDMS piston. The CSOW 110 is the device that controls the piston
Scheme of the CSOW 110 :
Overall dimensions of the CSOW 110:
The lid enables access to the petri dish, while confinement is performed allowing for instance to add or change cell medium.
The static cell confiner CSOW 110 kit is composed by:
- 1 x CSOW 110
- 2 x Confinement Piston in PDMS
- 10 x Confinement Slides – 16 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement
Available confinement heights – from 1, 2, 3, … up to 20 um (you can choose up to 3 heights to integrate your kit)
If you want to make your slides adhesive to your cells or not (optional):
- Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution
- Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides
If this device does not fit with your constraints, get in touch with us! We can personalize it for you!
CANCER
- Migration of metastatic cells
- Cell contractility in mestastasis
- DNA DSB repair (mechanically induced)
- Genomic instability (cell division)
- Separated co-culture
IMMUNOLOGY
- Migration of immune cells
- Imaging of non-adhesive cells
ORGAN PHYSIOLOGY
- Migration of cancer cells
- Cell differenciation with stiffness control
- Wound healing
- Separeted co-culture
- Cell compression response
RARE DISEASES
- Cell nucleus integrity
AGING
- Cell nucleus integrity
- Autophagy related diseases
OBSERVATION OPTIMIZATION
- Imaging of non-adhesive cells
- Planar imaging of organelles
FUNDAMENTAL RESEARCH
- Cell volume (cell cycle)
- Cell stretching response
Video of HeLa cells under confinement using a 4Dcell confiner, going from initial state to extremely confined.
Video of cells dividing under confinement using a 4Dcell confiner
Example of mammalian cells with before and after confinement images with fluorescent proteins
- Confinement and Low Adhesion Induce Fast Amoeboid Migration of Slow Mesenchymal Cells
Y.-J. Liu, M. Piel, Cell, et al., 2015 160(4), 659-672 - Actin flows induce a universal coupling between cell speed and cell persistence
P. Maiuri, R. Voituriez, et al., Cell, 2015 161(2), 374–386 - Geometric friction directs cell migration
M. Le Berre, M. Piel, et al., Physical Review Letter 2013 111, 198101 - Mitotic rounding alters cell geometry to ensure efficient spindle assembly
O. M. Lancaster, B. Baum, et al., Developmental Cell, 2013 25(3), 270-283 - Fine Control of Nuclear Confinement Identifies a Threshold Deformation leading to Lamina Rupture and Induction of Specific Genes
M. Le Berre, J. Aubertin, M. Piel, Integrative Biology, 2012 4 (11), 1406-1414 - Exploring the Function of Cell Shape and Size during Mitosis
C. Cadart, H. K. Matthews, et al., Developmental Cell, 2014 29(2), 159-169 - Methods for Two-Dimensional Cell Confinement
M. Le Berre, M. Piel, et al., 2014, Micropatterning in Cell Biology Part C, Methods in cell biology, 121, 213-29