Cell migration in cancer assay

Analysis of cell behaviors during metastasis

4DCELL DEVICE

Microchannels

 

READ-OUTS

Observation of cell motility and quantification of migration speed

 

STANDARD CULTURE LIMITATION

Cancer is considered a localized disease in its early stages; however, in the process of metastasis, cancer cells of a typical solid tumor must loosen their adhesion to neighboring cells, escape from the tissue of origin, invade other tissues by degrading the tissues extracellular matrix until reaching a blood or lymphatic vessel, to enter circulation, and proliferate in the new environment in which they ultimately reside. Those physiological constraints are difficult to reproduce with a standard cell culture.

 

CELL MIGRATION IN CANCER ASSAY

Using 4Dcell microchannels, natural constraints can be reproduced to mimic the trajectory of cancer cells spreading away from the primary tumor.

 

EXAMPLES

 

Motility of breast cancer cells in 3µm channels (filled circles) and in 12µm channels (empty squares) [2]

Motility can be measured and is better maintained with 12µm channels.

Motility of breast cancer in microchannels

Schematic representation of the motion of aggregated HeLa cells passing through a microcapillary to seed distant metastasis [2]

Live and metastatic cells can be observed after passing through a constriction.