Chemotaxis assay

Analysis of cell behaviors and cell migration in confined spaces

4DCELL DEVICE

Microchannels

 

READ-OUTS

Chemotaxis, chemo invasion, comparison of different cell type behaviors and cell migration in confined spaces

 

STANDARD CULTURE LIMITATION

Commonly used in vitro assays, such as the modified Boyden chamber or trans-well assay, provide end-point data but no information on cell behavior between the start and conclusion of the experiment.

 

CHEMOTAXIS ASSAY

With the 4Dcell assay, you can analyze the migration of many cell types after the establishment of a chemotactic gradient generated by positive pressure between inlet and outlet wells, that does not require the use of external pumps.

 

EXAMPLES

 

Dynamic optical range of microchannels for variable-scale analysis of cellular behavior [1]

Dynamic optical range of microchannels for variable-scale analysis of cellular behavior

Data can be obtained on many cells simultaneously (a), in a specific microchannel configuration (b) or from an individual cell (c).

Cell morphology and cell speed is Influenced by channels width [1]

Cell morphology and cell speed is Influenced by channels width

When channel width is over 20µm (a-b), cells are like in 2D flat surface(f) and they are not constricted. But, when the width decreases, the cells tends to be deformed (c-e).

In addition, under 20µm, cell speed decreases with the width (g).