Endocytosis assay

Improved observation of events taking place at the membrane

4DCELL DEVICE

Cell confiner

 

READ-OUTS

Observation of non-adherent cells, detection of the endocytosis events in adherent cells at the apical and basal membranes

 

STANDARD CULTURE LIMITATION

Observing endocytosis events in non-adherent cells, since cells cannot be observed in a common petri-dish, can be very challenging.

Using TIRF observation for adherent cells enables to study the mechanisms at the basal membrane making difficult the observation of apical membrane’s events.

 

ENDOCYTOSIS ASSAY

Using 4Dcell’s cell confinement, cells are confined between two surfaces enabling the study of non-adherent cells and an easier observation of the mechanisms at the membrane especially for clathrin-dependant endocytosis.

In addition, confining adherent cells enables to study endocytosis mechanisms at the basal membrane, as usual, but also at the apical membrane (confinement membrane).

 

EXAMPLES

Non-adherent cells are forced to spread on the bottom of the well [1]

Confined cells

Cell confinement for TIRFM acquisition at basal and apical membranes

Formation of endocytic clathrin-coated structures on the plasma membrane of mammalian cells [2]