Exocytosis assay

Improved observation of events taking place at the apical membrane

4DCELL DEVICE

Cell confiner

 

READ-OUTS

Detection of the exocytosis events in adherent cells at the apical membrane, TIRFM acquisition

 

STANDARD CULTURE LIMITATION

Observing exocytosis events at the apical membrane of adherent cells can be challenging by TIRFM, due to the distance between the apical membrane and the glass coverslip.

 

EXOCYTOSIS ASSAY

Using 4Dcell cell confinement system, cells are confined between two surfaces enabling an easy observation of the mechanisms at the apical membrane.

 

EXAMPLES

Non-adherent cells are forced to spread on the bottom of the well [1]

Confined cells

Cell confinement for TIRFM acquisition at apical membrane