Immune system in a well

2D migration and interaction of non-adherent immune cells

4DCELL DEVICE

Cell confiner

 

READ-OUTS

Attraction and interactions in-between cells

 

STANDARD CULTURE LIMITATION

Non-adherent immune cells poorly migrate on flat surfaces which can limit the analysis of their behavior.

 

IMMUNE SYSTEM IN A WELL BENEFITS

Confined in-between two surfaces, immune cells show restored migration behaviors and can migrate in a 2D space. This makes their observation and analysis with a microscope more easy than in within a 3D matrix. In these conditions, interaction in-between cells, which easily, meet are easy to analyse.

 

EXAMPLE

Migration of mice bone marrow derived dendritic cells (DCs) in fibronectin coated substrates [3]:

DCs in a confinement assay show a diffusion coefficients 5 times larger than when plated in common adhesive substrates

Confinement application

REFERENCES

[1] Liu, Y.-J et al. (2015). Cell, 160(4), 659–672.

[2] Maiuri, P., et al., Cell, 161(2), 374–386.

[3] Heuz, L., et al., Immunulogical Reviews, 256, 240–254