Quantitative cell migration assays

Fast and fine analysis of cell migration properties

4DCELL DEVICE

Microchannels (1D migration, confined)

 

READ-OUTS

Quantification of cell migration speed, persistence and diffusion coefficient.

 

STANDARD CULTURE LIMITATION

Non-adherent immune cells poorly migrate on flat surfaces which can limit the analysis of their behavior. In addition, the 2D random walk of a cell is difficult to analyse.

 

QUANTITATIVE CELL MIGRATION ASSAY

When confined, immune cells show restored migration behaviors and can migrate in 1D, 2D or 3D spaces. This makes their observation and analysis with a microscope more easy than in within a 3D matrix, enabling to quantify their exact cell speed, persistence and diffusion coefficient. 

 

EXAMPLE

Migration of DCs in a 400 um fibronectin coated microchannel. Measurement of speed for wild type DCs versus li (antigen) regulated DCs [2]:

Microchannels application