Quantitative cell migration assay
Fast and fine analysis of cell migration properties
Fast and fine analysis of cell migration properties
Quantification of cell migration speed, persistence and diffusion coefficient
All migrating cells (e.g. immune cells)
Non-adherent immune cells poorly migrate on flat surfaces, which can limit the analysis of their behavior. In addition, the 2D random walk of a cell is difficult to analyse.
When confined, immune cells show restored migration behaviors and can migrate in 1D, 2D or 3D spaces.
This makes their observation and analysis with a microscope easier than within a 3D matrix, enabling the quantification of their exact cell speed, persistence and diffusion coefficient.
Migration of Dendritic cells (DCs) in a 400 μm wide fibronectin coated microchannel. Measurement of speed for wild type DCs versus li (antigen) regulated DCs [2]:
[1] Maiuri, P., et al. Cell. 2015 Apr 9;161(2):374-86
[2] Faure-André, G., et al. Science. 2008 Dec 12;322(5908):1705-10