Quantitative cell migration assay

Fast and fine analysis of cell migration properties

4DCELL DEVICE

Microchannels 

 

READ-OUTS

Quantification of cell migration speed, persistence and diffusion coefficient

 

CELL TYPE

All migrating cells (e.g. immune cells)

 

STANDARD CULTURE LIMITATION

Non-adherent immune cells poorly migrate on flat surfaces, which can limit the analysis of their behavior. In addition, the 2D random walk of a cell is difficult to analyse.

 

QUANTITATIVE CELL MIGRATION ASSAY BENEFITS

When confined, immune cells show restored migration behaviors and can migrate in 1D, 2D or 3D spaces.

This makes their observation and analysis with a microscope easier than within a 3D matrix, enabling the quantification of their exact cell speed, persistence and diffusion coefficient. 

 

EXAMPLE

Migration of Dendritic cells (DCs) in a 400 μm wide fibronectin coated microchannel. Measurement of speed for wild type DCs versus li (antigen) regulated DCs [2]:

Microchannels application

REFERENCES

[1] Maiuri, P., et al.(2015). Cell, 161

[2] Gabrielle Faure-André, et al. (2008)  Science (New York, N.Y.) : 1705-10