SmartSphero 3D Organoid Plates
Why SmartSphero?
Traditional spheroid culture is a nightmare of lost samples, variable sizes, and data you can't trust. Organoids float away during medium changes. Manual positioning wastes hours. Imaging requires constant refocusing. The result is irreproducible experiments, rejected manuscripts, and research that stalls.
SmartSphero solves this. Our precision-engineered hydrogel platform creates thousands of uniform organoids that stay exactly where you put them, delivering the statistical power you need and the reproducibility reviewers demand. A major advantage of this assay is that the spheroids are anchored to the bottom of the microwells, preventing their loss during handling and making imaging easier, since all spheroids remain in the same focal plane compared to those kept in suspension.
What Makes SmartSphero Different
- Organoids That Actually Stay Put: Our patented APT (Anchoring Point Technology) locks each organoid at the bottom of conical microwells. No more lost samples during medium changes. No more frantically searching for floating spheroids. Just stable, trackable organoids from day 1 to final analysis.
- Organoids on the Same Focal Plane: Every single organoid sits at identical z-height. Image an entire well in seconds without refocusing. This isn't just convenient, it's transformative for high-throughput screening and automated imaging.
- >95% Uniformity Guaranteed: Engineered microwell geometry controls organoid size and shape with precision traditional methods can't match. CV values consistently below 10%. This is the reproducibility that gets papers accepted.
- Up to 5,280 Organoids Per Plate: Need statistical power for low-signal assays? Generate up to 5,280 independent organoids per plate. Run robust dose-response curves, detect subtle drug effects, and generate publication-quality statistics from a single experiment.
- Zero Special Equipment Required: Works with your existing microscope. Compatible with your current liquid handlers. Fits your standard plate readers. SmartSphero drops seamlessly into workflows you're already using.
- Automation-Ready From Day One: Standard plate formats (96-well to 384-well). Integrate with robotic liquid handlers, high-content imagers, and screening platforms without modification. Scale from bench research to industrial throughput on the same platform.
- Optically Transparent Hydrogel: Crystal-clear imaging through the entire organoid. #1.5 glass-bottom design works perfectly for brightfield, fluorescence, confocal, and high-content analysis. No optical artifacts. No autofluorescence. Just clean data.
- Culture for Weeks, Not Days: Stable long-term culture for maturation studies, chronic drug exposure, and developmental biology. Organoids maintain viability >85% for 15+ days without detachment or drift.
How It Works
- Cell-Repellent Hydrogel Microwells: Anti-adhesive properties facilitate rapid self-assembly of cell aggregates into compact spheroids. Cells naturally organize without manual intervention.
- Patented APT Contact Points: Strategic anchoring points secure each spheroid in a predictable location on the same focal plane. The hydrogel remains optically transparent for flawless microscopy.
- Inclined Microwell Walls: Engineered geometry guides cells to the anchoring point and controls organoid size with >95% reproducibility. Every organoid forms identically.
- Just Plate, Assemble, Measure: Plate your cells. Let them self-assemble. Culture normally. No special handling. No organoid loss during medium exchanges. Track individual organoids over weeks with perfect spatial consistency.
Applications
Drug Discovery & Toxicology
- High-throughput compound screening with 5,000+ data points per plate
- Precise dose-response curves with robust technical replicates
- Multi-endpoint analysis without sample transfer
- Chronic exposure studies with stable long-term culture
Disease Modeling
- Patient-derived organoids with consistent phenotypes
- Tumor spheroid growth and drug response
- Kidney, liver, brain, and gut organoid models
- Co-culture systems for tumor microenvironment studies
Academic & Translational Research
- Developmental biology with time-lapse tracking
- Stem cell differentiation with spatial control
- Host-pathogen interaction studies
- Organoid biobanking and phenotyping
Quality Control & Bioproduction
- Cell line characterization and validation
- Batch-to-batch consistency testing
- Potency assays for cell therapies
- 3D spheroid-based bioassays
Technical Specifications
- 24-well and 96-well configurations available
- Up to 5,280 uniform organoids per plate
- #1.5 glass-bottom for optimal imaging
- SLAS/ANSI standard for automation
ROI That Actually Matters
SmartSphero delivers lower cost per reliable data point through:
- Zero organoid loss saves expensive reagents and precious cells
- 95% reproducibility eliminates failed experiments and repeat studies
- Automation compatibility slashes labor costs
- Single focal plane reduces imaging time by 70%
Documentation for Validation
✓ GLP-compatible validation data
✓ Batch-to-batch consistency reports
✓ Reproducibility across instruments
✓ Quality control specifications