> EXPLORE CELL MECHANICS

6-WELLS STATIC CONFINER WITH CO2 PLUG – CSOW 620

6 wells confiner CO2 chamber

The CSOW 620 – static confiner is a portable device that allows confining cells within two surfaces with nanometer precision. The space between the two surfaces is controlled by using micro PDMS pillars. The micro pillars are fabricated in a glass slide, which is attached to a PDMS piston.
The CSOW 620 is the device that controls the position of the pistons and it is compatible with several brands of 6-well plates.  In the end of the confinement experiments, the cells can be retrieved enabling further biochemical studies. The user decides when and where to confine the cells as it is not necessary to plug it to any other device. In particular, the CSOW 620 is characterized by its compact design and CO2 plug.

 

> Define the thickness and shape of cells 

Control the thickness of your cells with the right confinement slide

 

> Multiple experiments simultaneously

Enables the study of different cells or to apply different confinement conditions at the same time

 

> Adaptable with high resolution microscopy

Optically transparent materials and compact design enable high resolution microscopy

> THE STATIC CELL CONFINER CSOW 110 IS COMPOSED BY:

1 x CSOW 620

12 x Confinement Piston in PDMS

12 x Confinement Slides – 16 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement

Available confinement heights – from 1, 2, 3, … up to 20 um (you can choose up to 3 heights to integrate your kit)

 

> IF YOU WANT TO MAKE YOUR SLIDES ADHESIVE TO YOUR CELLS OR NOT (OPTIONAL):

Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution

Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides

 

If this device does not fit with your constraints, get in touch with us! We can personalize it for you!

> SCHEME OF THE CSOW 110

CSOW620_2
CSOW620_3

Overall dimensions of the CSOW 620

CSOW620_1

> TO STUDY CELL DYNAMICS TRIGGERED BY/UNDER MECHANICAL EFFECTS

Migration

Cell division

Induce autophagy

Mechanotransduction

Mechanics of the nucleus

 

> HIGHT RESOLUTION IMAGING

 

> CO-CULTURE OF CELLS

Video of HeLa cells under confinement using a 4Dcell confiner, going from initial state to extremely confined.

Video of cells dividing under confinement using a 4Dcell confiner

Example of mammalian cells with before and after confinement images with fluorescent proteins

Confinement and Low Adhesion Induce Fast Amoeboid Migration of Slow Mesenchymal Cells
Y.-J. Liu, M. Piel, Cell, et al., 2015 160(4), 659-672

 

Actin flows induce a universal coupling between cell speed and cell persistence
P. Maiuri, R. Voituriez, et al., Cell, 2015 161(2), 374–386

 

Geometric friction directs cell migration
M. Le Berre, M. Piel, et al., Physical Review Letter 2013 111, 198101

 

Mitotic rounding alters cell geometry to ensure efficient spindle assembly
O. M. Lancaster, B. Baum, et al., Developmental Cell, 2013 25(3), 270-283

 

Fine Control of Nuclear Confinement Identifies a Threshold Deformation leading to Lamina Rupture and Induction of Specific Genes
M. Le Berre, J. Aubertin, M. Piel, Integrative Biology, 2012 4 (11), 1406-1414

 

Exploring the Function of Cell Shape and Size during Mitosis
C. Cadart, H. K. Matthews, et al., Developmental Cell, 2014 29(2), 159-169

 

Methods for Two-Dimensional Cell Confinement
M. Le Berre, M. Piel, et al., 2014, Micropatterning in Cell Biology Part C, Methods in cell biology, 121, 213-29