EXPLORE CELL MECHANICS

6-WELLS STATIC CONFINER WITH CO2 PLUG – CSOW 620

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  • Define the thickness and shape of cells 

    Control the thickness of your cells with the right confinement slide

  • Multiple experiments simultaneously

    Enables the study of different cells or to apply different confinement conditions at the same time

  • Adaptable with high resolution microscopy

    Optically transparent materials and compact design enable high resolution microscopy

The CSOW 620 – static confiner is a portable device that allows confining cells within two surfaces with nanometer precision. The space between the two surfaces is controlled by using micro PDMS pillars. The micro pillars are fabricated in a glass slide, which is attached to a PDMS piston. The CSOW 620 is the device that controls the position of the pistons and it is compatible with several brands of 6-well plates.  In the end of the confinement experiments, the cells can be retrieved enabling further biochemical studies. The user decides when and where to confine the cells as it is not necessary to plug it to any other device. In particular, the CSOW 620 is characterized by its compact design and CO2 plug.

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Features

Overall dimensions of the CSOW 620:

CSOW620_1

Scheme of the CSOW 620:

CSOW620_2
CSOW620_3

The static cell confiner CSOW 620 kit is composed by:

  • 1 x CSOW 620
  • 12 x Confinement Piston in PDMS
  • 12 x Confinement Slides – 16 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement

Available confinement heights – from 1, 2, 3, … up to 20 um (you can choose up to 3 heights to integrate your kit)

If you want to make your slides adhesive to your cells or not (optional):

  • Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution
  • Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides

 

If this device does not fit with your constraints, get in touch with us! We can personalize it for you!

Applications

  • To study cell dynamics triggered by /under mechanical effects:
    • Migration
    • Cell division
    • Induce autophagy
    • Mechanotransduction
    • Mechanics of the nucleus
  • High resolution imaging
  • Co-culture of cells
More applications

Example of use

Video of HeLa cells under confinement using a 4Dcell confiner, going from initial state to extremely confined.

Video of cells dividing under confinement using a 4Dcell confiner

Example of mammalian cells with before and after confinement images with fluorescent proteins

Publications

  • Confinement and Low Adhesion Induce Fast Amoeboid Migration of Slow Mesenchymal Cells
    Y.-J. Liu, M. Piel, Cell, et al., 2015 160(4), 659-672
  • Actin flows induce a universal coupling between cell speed and cell persistence
    P. Maiuri, R. Voituriez, et al., Cell, 2015 161(2), 374–386
  • Geometric friction directs cell migration
    M. Le Berre, M. Piel, et al., Physical Review Letter 2013 111, 198101
  • Mitotic rounding alters cell geometry to ensure efficient spindle assembly
    O. M. Lancaster, B. Baum, et al., Developmental Cell, 2013 25(3), 270-283
  • Fine Control of Nuclear Confinement Identifies a Threshold Deformation leading to Lamina Rupture and Induction of Specific Genes
    M. Le Berre, J. Aubertin, M. Piel, Integrative Biology, 2012 4 (11), 1406-1414
  • Exploring the Function of Cell Shape and Size during Mitosis
    C. Cadart, H. K. Matthews, et al., Developmental Cell, 2014 29(2), 159-169
  • Methods for Two-Dimensional Cell Confinement
    M. Le Berre, M. Piel, et al., 2014, Micropatterning in Cell Biology Part C, Methods in cell biology, 121, 213-29
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