Single cardiomyocyte contractility force assay
Quantification of beating force of single cardiomyocyte cells
4DCELL DEVICE
READ-OUTS
Uniaxial Contraction Force, Analysis of Calcium Transients coupled to force measurements.
STANDARD CULTURE LIMITATION
When hESC-Cardiomyocyes are cultured in standard conditions, i.e., in 2D monolayers of cells, these are organized in a complete random way. Therefore, it is not possible to precisely quantify the uniaxial force generated by the beating of a single cardiomyocyte cell.
SINGLE CARDIOMYOCYTE CONTRACTILITY FORCE ASSAY BENEFITS
In this assay, derived cardiomyocytes attach to rectangular cell-adhesive areas, which induce cell elongation and promote suspended cell anchoring between two adjacent micropillars. The contractility force assay enables in vitro reproduction of excitation−contraction coupling effect responsible for the beating of CMs.
EXAMPLE
Quantification of calcium transients (activation of calcium-sensitive contractile proteins) and simultaneous measurement of cell contraction and force generation (analysis of pillars deflection) in hESC-Cardiomyocytes [1].
