Single cardiomyocyte contractility force assay 

Quantification of beating force of single cardiomyocyte cells

4DCELL DEVICE

Micropillars

 

READ-OUTS

Uniaxial Contraction Force, Analysis of Calcium Transients coupled to force measurements.

 

STANDARD CULTURE LIMITATION

When hESC-Cardiomyocyes are cultured in standard conditions, i.e., in 2D monolayers of cells, these are organized in a complete random way. Therefore, it is not possible to precisely quantify the uniaxial force generated by the beating of a single cardiomyocyte cell.

 

SINGLE CARDIOMYOCYTE CONTRACTILITY FORCE ASSAY

In this assay, derived Cardiomyocytes attach to rectangular cell-adhesive areas, which induce cell elongation and promote suspended cell anchoring between two adjacent micropillars. The contractility force assay enables in vitro reproduction of excitation−contraction coupling effect responsible for the beating of CMs.

 

EXAMPLE

Quantification of Calcium transients  (activation of calcium-sensitive contractile proteins) and simultaneous measurement of cell contraction and force generation (analysis of pillars deflection) in hESC-Cardiomyocyes [1].

Micropillars application