Micropatterned 96- and 384-Well Plates for High-Content Cell Assays
SmartPattern micropatterned multiwell plates are glass-bottom 96- or 384-well plates containing defined cell-adhesive patterns. The patterns control cell adhesion, shape and spatial organization, creating comparable geometrical conditions across wells for quantitative imaging and cell-based assays.
The plates are designed for single-cell or multicellular imaging, high-content screening, dose–response studies and automated workflows. Standard or customized pattern geometries can be selected according to the cell type, assay and imaging requirements.
SmartPattern micropatterned multiwell plate. Defined cell-adhesive geometries are arranged on the glass bottom of each well to control cell shape and spatial organization for quantitative imaging and high-content assays.
WHY USE MICROPATTERNED MULTIWELL PLATES?
On conventional culture surfaces, cell shape, spreading area and position vary from one cell to another. This geometrical variability can complicate quantitative imaging and the comparison of cellular phenotypes across experimental conditions.
SmartPattern plates confine cell adhesion to predefined micrometer-scale geometries, creating more comparable conditions across cells, wells and experiments while maintaining compatibility with multiwell workflows.
Create Comparable Cell Geometries
Control cell shape, spreading area, orientation and spatial organization using defined adhesive patterns.
Support Quantitative Imaging
Organize single cells or multicellular structures at predefined positions to facilitate image acquisition and phenotypic comparison.
Scale to Multi-Condition Assays
Use 96- or 384-well formats for experimental replicates, dose–response studies, high-content imaging and automated workflows.
Best suited for: high-content imaging, phenotypic screening, dose–response studies, multi-condition assays and automated workflows.
PRODUCT SPECIFICATIONS
Specification
Plate formats
Culture surface
Plate standard
Pattern geometries
Pattern design
ECM coating options
Cell organization
Imaging
Automation
Optical properties
Primary workflows
SmartPattern Multiwell Plates
96-well and 384-well plates
Glass bottom with defined cell-adhesive micropatterns surrounded by an anti-adhesive surface
SBS-format plates
Disks, lines, triangles, squares, rectangles and grids
Standard or customized geometries
Available pre-coated with selected ECM proteins or without an ECM protein coating for user-defined functionalization. Compatible options include fibronectin and collagen.
Suitable for individual cells or organized multicellular groups
Compatible with high-resolution optical imaging and commercially available image-analysis software
Compatible with robotic liquid-handling workflows
Glass imaging surface with low autofluorescence
Quantitative imaging, high-content screening, dose–response studies and multi-condition assays
STANDARD MICROPATTERNED PLATE LAYOUT
The standard plate layout provides defined micropattern geometries and dimensions across the wells, allowing researchers to compare different cell-confinement conditions within the same plate.
Standard layout of a SmartPattern 96-well micropatterned plate, showing the distribution of pattern geometries and dimensions across the plate.
Representative microscopy images of cells cultured on SmartPattern adhesive micropatterns of different shapes and sizes. Cell images are paired with fluorescence images of the corresponding circular, square, rectangular, linear, Y-shaped, grid and triangular geometries.
HOW TO USE SMARTPATTERN MULTIWELL PLATE
SmartPattern multiwell plates are ready to use.
Pre-coated SmartPattern® multiwell plates are ready for cell seeding. Plates supplied without an ECM protein coating require functionalization with a compatible adhesion protein before use. Cells are then seeded onto the micropatterned surface, allowed to adhere and washed to remove non-adherent cells before culture, imaging and analysis.
Cell seeding density, adhesion time and washing conditions should be optimized according to the cell type, pattern geometry and experimental workflow.