SmartPATTERN®
Dynamic micropatterned coverslips
Also available
in multiwell plates
24 mm round 'dynamic' micropatterned coverslips, multiwell plates, or dishes are ready to be applied in cell culture experiments.
They are ready to use, you just need to plate your cells on the glass substrate, wait for them to be patterned and observe them migrating on the substrate.
ā
-
Ready to use
Plate the cells on the patterned coverslip and observe them directly
-
High quality cell imaging
Observe the cells through the optically transparent micropatterned glass substrate
-
Very stable coating
The covalent bonds formed between the anti-adhesive coating and the glass coverslip enable storing for several months at room temperature
-
Inductible coating made possible by bioorthogonal click chemistry
The cells are first patterned in cell-adhesive regions and do not adhere outside of these regions. The surrounding anti-adhesive regions can be rendered adhesive by adding a single reagent onto the substrate : this induces cell migration into the newly adhesive regions.
How to handle your 'dynamic' micropatterned coverslips
Features & Benefits
4Dcell 'dynamic' micropatterned coverslips are shipped in individual classic 35 mm Petri dishes in plastic bags (aseptic conditions).
Available in sets of 20 or 50 units to allow optimization of your experimental conditions with only one kit.
You can choose various micropatterned shapes to compose your set.
Compatible with high resolution microscopy, phase contrast, immunofluorescence, etc.
The coverslips may be combined with perfusion chamber.
ā
Micropatterns standard shapes available:
-
Disks
-
Lines
-
Triangles
-
Squares
-
Rectangles
-
Grids
-
Customized shapes
Each type of micropatterned coverslip (disks, rectangles, squares, etc.) is arranged as described in the scheme bellow (e.g.: disks).
In each standard coverslip, there are seven zones with distinct pattern sizes (ranging from 10 µm to 100 µm). There is an eighth zone where all the sizes are placed together.
Also available
in multiwell plates
Standard positionning of micropatterned areas
A highly stable non-adhesive covalent coating
Long term cell culture anti-adhesive polymer which is the best in terms of anti-fouling treatment and bonding to the glass slide. Observable by phase contrast microscopy. This is the best option for long term cell culture on micropatterns (more than a week of cell culture).
ā
A highly efficient reagent for rendering the coating adhesive
The reagent for switching the coating from ‘non-adhesive’ to ‘ adhesive’ and activate cell migration is shipped in aliquots of 50 ug lyophilized powder. Once dissolved in its appropriate solvent, it can be stored at -20°C
SMARTPATTERN CATALOG
