MICROCHANNELS FABRICATION KIT

Make your own microchannels for cell assays

  • Control over the protocol

    All the tools needed to do chips by yourself

  • Various microchannels designs

    Choose 4 molds between small, medium and large microchannels designs

  • Autonomy and affordable experiment

    Make 100 microchannels chips and keep the knowledge in your lab.

Cells are loaded into a central chamber communicating with multiple microchannels that can be analyzed simultaneously by microscopy.
Suitable for Live Cell Imaging: compatible with high resolution live microscopy

Microchannels’ Dimensions

The 3D shape and stress conditions imposed to your cells depend on the microchannels size. Each chip has 6 ranges of microchannels and 9 access ports.

microchannels designs
small microchannels cell migration

Small microchannels chip design available in different heights (MC001, MC002, MC003, MC004)

medium microchannels cell migration

Medium microchannels chip design (MC005)

4Dcell also provides microchannels with constrictions, with the following sizes:

constriction design microchannels
constriction microchannels
constriction microchannels

Example of a 4Dcell microchannel with a constriction of 1.5 µm width.

What do you need to make your own Microchannels ?

To manufacture 100 chips, each 4DCell microchannels kit contains:

  • Silicone precursors to make 100 chips
  • 2 graduated plastic cups to measure the quantity of elastomer needed
  • A glass spatula to homogenize the PDMS with its curing agent
  • 4 individual molds to shape the silicone into the upper part of the chip containing the microchannels
  • Scalpel to unmold the chip
  • Thin glass bottom 35 mm petri dishes to make the lower part of the microchannels, and to observe cells with a microscope
  • A punches round to cut 3 mm holes into the chip
  • Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin)
  • A Plasma cleaner to activate the chip and allow its bonding to the glass

The mold on which the microchannels shapes are defined is the key element.
Several series of shapes are available, for migration assay with various sizes, shapes and profiles.
We put our expertise at your disposal to help you make your choice.

Which kit fits your needs ?

  • If you never made any microchannels:
    Microchannels Station (microchannels box + plasma cleaner)
  • If you already have a binding tool (for example a plasma cleaner):
    Microchannels Box (supplies box + microchannels mold)
  • If you have all the equipment but want to access the best chemicals:
    Supplies Box (silicone precursors, glass bottom petri dishes and solutions)

Each element can also be ordered separately.

The Microchannels provide a wide range of applications:

CANCER

  • Migration of metastatic cells
  • Nuclear plasicity in mestastasis
  • DNA DSB repair (mechanically induced)
  • Angiogenesis
  • Immuno-oncology

 

IMMUNOLOGY

  • Cell-cell interaction
  • Migration of immune cells
  • Antigene searching
  • Extravasation/diapedesis
  • Chemotaxis/haptotaxis

 

ORGAN PHYSIOLOGY

  • Migration of cancer cells
  • Shear stress
  • Angiogenesis
  • Epithelium permeability/brain blood barrier
  • Neural network

 

RARE DISEASES

  • Cell nucleus integrity
  • Nuclear plasticity
  • Neural network

 

FUNDAMENTAL RESEARCH

  • Cell adhesion

Example of iBMD Cells migrating within microchannels of different sizes (0.5 image/min)
Réf. video by 4Dcell partner Dr Pablo Vargas, Curie Institute/UMR144