Frustrated phagocytosis
Characterization of the frustrated phagocytosis mechanism
4DCELL DEVICE
READ-OUTS
Characterization of frustrated phagocytosis, evaluation of the interaction between lysosomes and frustrated phagosomes.
CELL TYPE
Phagocytic cells (e.g. macrophages, neutrophils, dendritic cells, mast cells).
STANDARD CULTURE LIMITATION
Frustrated phagocytosis cannot be properly observed in a 2D culture due to the 3D process of phagosome formation.
ASSAY BENEFITS
Using 4Dcell’s assays, molecular mechanisms regulating cellular processes of frustrated phagocytosis can be studied.
On micropatterns, macrophages can target opsonized molecules which are linked to micropatterns, thereby inducing this process [1].
When confined in a small volume, the cell is forced to be spread on the pattern [2].
EXAMPLES
Frustrated phagocytosis after opsonization on micropatterns
Frustrated phagosomes formation was observed on RAW cells after opsonization in micropatterns (A), whereas no phagosomes could be detected in the absence of opsonization (B). Human macrophages derived from monocytes (HMDM) were also able to form fp (C) [1].