Frustrated phagocytosis

Characterization of the mechanism

4DCELL DEVICE

Confiner; Micropatterns

 

READ-OUTS

Characterization of frustrated phagocytosis, evaluation of the interaction between lysosomes and frustrated phagosomes.

 

CELL TYPE

Phagocytic cells (e.g. macrophages, neutrophils, dendritic cells, mast cells).

 

STANDARD CULTURE LIMITATION

Frustrated phagocytosis cannot be properly observed in a 2D culture due to the 3D process of phagosome formation.  

 

FRUSTRATED PHAGOCYTOSIS ASSAY BENEFITS

Using 4Dcell’s assays, molecular mechanisms regulating cellular processes of frustrated phagocytosis can be studied.

On micropatterns, macrophages can target opsonized molecules which are linked to micropatterns, thereby inducing frustrated phagocytosis [1]

When confined in a small volume, the cell is forced to be spread on the pattern [2].

 

EXAMPLES

Frustrated phagocytosis after opsonization on micropatterns

Frustrated phagosomes formation was observed on RAW cells after opsonization in micropatterns (A), whereas no phagosomes could be detected in the absence of opsonization (B). Human macrophages derived from monocytes (HMDM) were also able to form frustrated phagosocytosis (C)  [1].