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5000+ ORGANOIDS

5000+ ORGANOIDS

5000+ ORGANOIDS

4DCELL NAMs DESIGN OFFICE

One Platform Strategy, Unlimited NAM Possibilities

4Dcell leverages its two proven technology platforms to design and deliver human-relevant New Approach Methodologies (NAMs) for a broad range of non-cardiac in vitro applications.

By combining SmartContractility™ and SmartSphero™, we address virtually any NAM challenge, whether involving contractile tissues or non-contractile organoid systems. These platforms enable the generation of robust, reproducible, and decision-ready human models, precisely tailored to your biological question.

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Our Design Office integrates these technologies with custom endpoints, exposure paradigms, and analytical workflows to support safety, toxicology, efficacy, and mechanism-of-action studies, working in close scientific collaboration with our clients.

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CONTRACTILE ORGANOID MODELS

Powered by SmartContractility™ Technology

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Our SmartContractility™ platform is a dedicated solution for contractile human tissue models, extending beyond the heart to any tissue where force generation and dynamic function are key biological drivers.

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Applications include

  • Skeletal muscle models

  • Smooth muscle models

  • Uterine and reproductive tissue models

  • Other force-generating or mechanically active tissues

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Key strengths

  • Quantitative force and functional readouts

  • Controlled geometry and tissue maturation

  • High reproducibility across conditions and studies

  • Suitable for acute and chronic exposure designs

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These models are particularly well suited for efficacy testing, safety profiling, and mechanism-of-action studies where functional performance is a critical endpoint.

NON CONTRACTILE ORGANOID MODELS

Powered by SmartSphero™ Technology

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Our SmartSphero™ platform is a versatile organoid-forming technology enabling the generation of stable, homogeneous, and reproducible 3D organoids from human cells.

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Applications include

  • Non-contractile tissue and organ models

  • Disease-relevant organoids

  • Multi-cellular and complex biological systems

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Key strengths

  • Robust organoid formation with low variability

  • Scalable and assay-ready formats

  • Compatible with functional, molecular, and imaging readouts

  • Adaptable to a wide range of tissues and indications

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SmartSphero™ enables custom NAM development for projects where structure, cellular organization, and molecular responses are central to biological relevance.​​

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Design Office Services

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1. Custom Study Design & Experimental Strategy


Our Design Office supports the conception, optimization, and execution of tailored in vitro studies beyond cardiac applications. We collaborate closely with your scientific team to define the most relevant biological models, endpoints, and experimental workflows aligned with your project objectives.

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This service is ideal when:

  • Standard assays do not fully address your biological question

  • You need a customized multi-readout strategy

  • You are exploring a new target, modality, or indication

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Typical applications

  • Safety and toxicity screening (non-cardiac tissues)

  • Proof-of-concept and efficacy studies

  • Mechanism-of-action investigations

  • Assay transfer, optimization, or feasibility studies

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2. Assay Development & Optimization

 

We develop and optimize bespoke in vitro assays, from early feasibility to ready-to-run screening formats. This includes biological model selection, protocol refinement, and definition of robust readouts.

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Capabilities

  • Selection of relevant human cell models (2D, 3D, co-culture)

  • Endpoint definition (functional, molecular, imaging-based)

  • Protocol optimization for reproducibility and robustness

  • Scalability assessment for screening or longitudinal studies

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3. Readouts & Deliverables

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Depending on project scope

  • Functional endpoints (cell behavior, viability, activity)

  • Molecular readouts (qPCR, RNA-seq, biomarker panels)

  • Imaging and morphological analysis

  • Integrated datasets with scientific interpretation

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​Deliverables

  • Study design proposal and experimental plan

  • Processed data and clear summary reports

  • Optional raw data and protocols

  • Scientific recommendations for next steps

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Why Use Our Design Office?

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  • Biology-driven, not catalog-driven

  • Human-relevant in vitro focus

  • Flexible, project-specific approach

  • Direct interaction with experienced scientists

DO YOU WANT TO PUSH THE LIMITS OF 3D CELL CULTURE? LET'S DISCUSS YOUR PROJECTS

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