DYNAMIC CONFINER

A step towards mimicking the natural cell environment

  • Control the confinement speed

    Accurate control of the confinement speed through the vacuum pump

  • Reversible confinement: retrieve your cells after confinement

    Allows molecular analysis thanks to the non-destructive method for the cells

  • Compatible with your own experiments

    The confiner is a small size device that is directly placed on the top of your cell culture

The dynamic cell confiner is a device that allows confining cells within two surfaces with micrometer precision. The space between the two surfaces is controlled by micro PDMS pillars. The micro pillars are fabricated in a glass slide, which is attached to a PDMS piston (suction cup). The piston is controlled with a vacuum pump, and the height of the confinement is thus controlled as well. The device can be used in a petri dish, in a glass substrate, etc.

The dynamic cell confiner kit is composed by:

  • 1 x Elveflow Vacuum & Pressure Generator and 1 x Elveflow AF1 DUAL – Vacuum & Pressure Controller
  • 10 x Confinement Slides – 10 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement.
    Available confinement heights – from 1, 2, 3, … up to 20 um (choose up to 3 heights to integrate your kit)
  •  3 x 4Dcell Dynamic Confiner™ (PDMS Suction Cup)
  •  1 x security tank (to preserve the vacuum pump)
  •  1 x Kit of Tubes and Connectors

If you want to make your slides adhesive to your cells or not (optional):

  • Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution
  • Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides

CANCER

  • Migration of metastatic cells
  • Cell contractility in mestastasis
  • DNA DSB repair (mechanically induced)
  • Genomic instability (cell division)
  • Separated co-culture

 

IMMUNOLOGY

  • Migration of immune cells
  • Imaging of non-adhesive cells

 

ORGAN PHYSIOLOGY

  • Migration of cancer cells
  • Cell differenciation with stiffness control
  • Wound healing
  • Separeted co-culture
  • Cell compression response

 

RARE DISEASES

  • Cell nucleus integrity

 

AGING

  • Cell nucleus integrity
  • Autophagy related diseases

 

OBSERVATION OPTIMIZATION

  • Imaging of non-adhesive cells
  • Planar imaging of organelles

 

FUNDAMENTAL RESEARCH

  • Cell volume (cell cycle)
  • Cell stretching response

Video of HeLa cells under confinement using a 4Dcell confiner, going from initial state to extremely confined.

Video of cells dividing under confinement using a 4Dcell confiner

Example of mammalian cells with before and after confinement images with fluorescent proteins
Le Berre M, Aubertin J, Piel M., Fine control of nuclear confinement identifies a threshold deformation leading to lamina rupture and induction of specific genes. Integr Biol (Camb). 2012 Nov;4(11):1406-14.