SmartCONFINEMENT®
Dynamic Confiner 1 well
The dynamic cell confiner is a device that confines cells between two parallel surfaces to a defined micrometer height with sub-micrometer precision. The space between the two surfaces is controlled by micro PDMS pillars. The micropillars are fabricated on a glass slide, which is attached to a PDMS piston (suction cup). The piston is controlled with a vacuum pump, and the height of the confinement is thus controlled as well.
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Control the confinement speed
Accurate control of the confinement speed through the vacuum pump
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Reversible confinement: retrieve your cells after confinement
Allows molecular analysis thanks to the non-destructive method for the cells
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Compatible with your own experiments
The confiner is a small size device that is directly placed on the top of your cell culture
How to set up your 1-well dynamic confiner
Features & Benefits
The dynamic cell confiner kit is composed of:
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1 x Autonomous vacuum pump
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12 x Confinement Slides – 10 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement.
Available confinement heights – from 1, 2, 3, … up to 20 µm (choose up to 3 heights to integrate your kit)
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3 x 4Dcell Dynamic Confiner™ (PDMS Suction Cup)
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1 x Security tank (to preserve the vacuum pump)
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1 x Kit of Tubes and Connectors
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3 x PDMS ring shaped stencil – delimits the seeding area of cells to the region where confinement is applied
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If you want to make your slides adhere to your cells or not (optional):
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Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution
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Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides
