SmartCONFINEMENT®

Static Confiner 1 well

The 1-well static confiner is a portable device that confines cells between two surfaces to a defined micrometer height with nanometer precision. The space between the two surfaces is controlled by using micro PDMS pillars. The micropillars are fabricated on a glass slide, which is attached to a PDMS piston.

The CSOW 110 is the device that controls the piston mechanically and it is compatible with glass bottom petri dishes (35 mm). The cells can be retrieved after confined, enabling further biochemical analysis. The user decides when and where to confine the cells as it is not necessary to plug it to any other device.

User friendly and convenient

It is easy to assemble and is portable

Enables high resolution imaging

It fits with glass bottom petri dishes (35 mm) and the observation area is made of optically transparent materials

Adaptable with your microscope

It was designed to fit in your microscope

How to set up your 1-well static confiner

Features & Benefits

The static cell confiner CSOW 110 kit is composed of:

1 x CSOW 110
12 x Confinement Slides – 16 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement

Available confinement heights – from 1, 2, 3, … up to 20 µm (you can choose up to 3 heights to integrate your kit)

2 x Confinement Piston in PDMS

If you want to make your slides adhere to your cells or not (optional):

Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution
Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides

If this device does not meet your needs, get in touch with us! We can personalize it for you.

Video protocol to set up perfectly your CSOW 110

Anatomy of the CSOW 110