3D Liver canaliculi assays

Spheroid formation in 3D gels

4DCELL DEVICE

Coverslips coated with gels

 

READ-OUTS

Identification, quantification and analysis of bile canaliculi

 

STANDARD CULTURE LIMITATION

Cultured in a 2D environment, cells loose rapidly their phenotypic expression and their functions.

 

3D LIVER CANALICULI ASSAYS

A 3D environment is more relevant for cell culture because it enables to reflect in vivo physiology of cells.
4Dcell offers soft 3D gels for the fulfilment of a cell culture enabling spheroid formation to better mimic in vivo conditions.

 

EXAMPLE

Accumulation of F-actin, MRP2 and MDR1, specific markers of the canalicular plasma membrane, have been detected in low-density HepaRGTM cultures using wood-derived nanofibrillar cellulose (NFC) and hyaluronan-gelatin (HG) hydrogels after spheroid formation. It has been compared to a 2D cell culture where the activity of MRP2 and MDR1 is lower.

Spheroid formation in a soft substrate versus flat substrate