Printing the micropatterns:
The main application of the UV Printer related with our products is to print the micropatterns onto the glass substrate (either a Micropatterned slide or a Micropatterned dish).The lamp and everything you need to get micropatterns on slides is provided with the Micropatterns Fabrication Kit. The following brief procedure will illustrate the use of the UV Printer.
First, the glass substrate is coated with an anti-adhesive agent, e.g. Polyethylene glycol (PEG) (pre-coated slides can be provided).
To pattern the surface, a Micropatterning Photomask is placed over the substrate. It will define the areas that will enable the cells to be adherent.
Then, the high energy light of the deep UVs will damage the exposed areas printing the patterns.
Finally, extra cellular matrix (ECM) proteins are loaded over the treated surface. The anti-fouling effect of the patterned coating will make the ECM attach only on the damaged areas. Thus, the cells will adhere and multiply only over the restricted areas.