> MICROPATTERNED COVERSLIPS

Patterned slides for immunofluorescence observation

micropatterned slides

4Dcell offers 24 mm round micropatterned coverslips ready to be applied in cell culture experiments. 

Several standard micropattern shapes available as triangles, lines, rectangles, squares, etc.  

 

> Ready to use

Plate the cells on the patterned coverslip and observe them directly

 

> Adaptable to your imaging system

Place your slide on the imaging system suitable for your study

 

> Very stable coating

The covalent bonds formed between the anti-adhesive coating and the glass coverslip enable storing for several months

4Dcell micropatterned coverslips are shipped in individual classic petri dishes in sterile plastic bags.

Sold in sets of 20 or 50 units. 

Compatible with high resolution microscopy, phase contrast, imonofluorescence, etc. 

For high resolution microscopy you can consider combining the coverslips with perfusion chambers. 

 

> MICROPATTERNS STANDARD SHAPES AVAILABLE:

 

disks

lines

triangles

squares

rectangles

grids

 

If 4Dcell standard shapes do not meet the conditions you want to mimic, please don not hesitate in getting in touch. We can customize the design!

 

Each type of micropatterned coverslip (disks, rectangles, squares, etc.) is arranged as described in the scheme bellow (e.g.: disks).

In each standard coverslip, there are seven zones with distinct pattern sizes (ranging from 10 µm to 100 µm). There is an eighth zone where all the sizes are placed together.

Standard positionning of micropattern areas

> 4DCELL OFFERS 3 OPTIONS OF COVALENT COATINGS:

 

Standard anti-adhesive polymer with the same anti-fouling effect than traditional PLL-g- PEG, but with a more stable bonding to the glass slide (covalent). After plated, you can keep you cells in the patterns for a few days. Non used coverslips can be stored for 1 month. 

Oxidation resistance anti-adhesive polymer which is less sensitive to oxidation and has the same type of covalent bonding to the glass slide. After plated you can keep your cells in the patterns for more than a week. Non used coverslips can be stored up to 6 months.

Long term cell culture anti-adhesive polymer which is the best in terms of anti-fouling treatment and bonding to the glass slide.

 

> CANCER

 

Migration of cancer cells (line patterns)

Cell shape standardization

 

> ORGAN PHYSIOLOGY

 

Migration of somatic and cancer cells

Cell shape standardization

Cardiomyocyte beating properties

Neural network

 

> RARE DISEASES

 

Cell nucleus integrity

Nuclear plasticity

Neural network

 

> FUNDAMENTAL RESEARCH

 

Cell shape standardization

Standardized connectivity between neurons

 

> BIOMOLECULAR IN VITRO MODELS

 

Spatial autoorganization of macromolecules (at the microscale)

Video of cells developing on the long term cell culture polymer micropatterns.
Dr Martial Balland, Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier.

micropatterns cardiomyocytes

hPSC-CM on line micropatterns of 10 to 70 µm width imaged by fluorescence microscopy.
Dussaud S., Jouve C., Hulot J.S., 2018.

HeLa cells micropatterns

Image of micropatterned HeLa cells in lines of 100 to 10 µm width, 4Dcell lab (2018).

line micropatterns

Image of micropatterned cells in lines (labelled by fluorescence) obtained with a 4Dcell photomask.
Hôpital Saint Louis, experiment run with Dr. Benoit Vianay.