Liver canaliculi assays

Improved hepatocytes differentiation model

4DCELL DEVICE

Micropatterns

 

READ-OUTS

Observation/quantification of biliary canaliculi

 

STANDARD CULTURE LIMITATION

The process of differentiation of HepaRG™ into hepatocytes is long, as it takes approximately two weeks to reach the necessary confluency of cells. In addition to this, as the cells are randomly organized, bile caniculae forming in between cells are difficult to be visualized and quantified.

 

LIVER CANALICULI ASSAYS

Cells are cultured in adhesive patterned substrates, having optimal sizes and shapes which leads to the organized formation of biliary caniculae between cells. This enables their easy observation/quantification.

 

EXAMPLE

Organization of HepaRG cells after two days of culture (a) in line micropatterns. Observation of bile caniculae (bright) formed in between hepatocytes differentiated from HepaRG (b). 

Micropatterned cells application