> MULTIWELL PLATE

96 or 384-well plates for high throughput screening and high content imaging of cell-based micropatterned assays

384-well plate

4Dcell micropatterned multi-well plates hold a perfectly organized and stable culture substrate aiming for cell normalization. Evenly distributed on adhesive patterns, cells have a controlled geometry allowing standardization of the assays.
4Dcell micropatterning technology enables 2D cell geometry control. A set of patterns is arranged on a glass bottom to receive cells. Using an anti-adhesive agent and some ECM proteins such as fibronectin or collagen, cells adhere and acquire the shape imposed by the micropattern.

 

> Imaging of single cells and organized cell groups

 

> Robot pipette ready / SBS 96 or 384-well plate

 

> High-resolution imaging. Compatible with imaging software commercially available

 

> Minimal auto-fluorescence

4Dcell micropatterned 96 well plates hold a patterned surface to control the 2D geometry of cells and standardize cellular assays.

Each 96-well plate has the 6 standard shapes of 4Dcell: dots, squares, rectangles, triangles, lines, and grids, as well as sizes going from 10 to 150 µm.

We also offer the choice of a customizable solution (additional shapes and dimensions).

The 4Dcell device is compatible with industrial standards for screening platforms (e.g. drug discovery).

The bottom of the 4Dcell plate is made in glass and is coated with a stable anti-adhesive polymer to delimitate the borders of the patterns. This coating is resistant to oxidation, can be kept at room temperature for six months, and enables keeping the cells in patterns for several days.

Map of the standard Micropatterned 96 well plate

> DRUG SCREENING

High throughput and high content screening

 

> ORGAN PHYSIOLOGY & DISEASE

Migration of somatic and cancer cells

Cell shape standardization

Cardiomyocyte beating properties

Neural network and neuromuscular junction assays

Cell nucleus integrity

Nuclear plasticity

Co-culture

Wound healing

Cellular division

Cell-cell contact and interaction

Cell polarization

Frustrated phagocytosis

Cellular membrane protrusion assays (lamellipodia and filipodia)

 

> BIOMOLECULAR IN VITRO MODELS

Spatial auto-organization of macromolecules and organelles (at the microscale)

Video of cells developing on the long term cell culture polymer micropatterns.
Dr Martial Balland, Laboratoire Interdisciplinaire de Physique, Université Joseph Fourier.

micropatterns cardiomyocytes

hPSC-CM on line micropatterns of 10 to 70 µm width imaged by fluorescence microscopy.
Dussaud S., Jouve C., Hulot J.S., 2018.

HeLa cells micropatterns

Image of micropatterned HeLa cells in lines of 100 to 10 µm width, 4Dcell lab (2018).

line micropatterns

Image of micropatterned cells in lines (labelled by fluorescence) obtained with a 4Dcell photomask.
Hôpital Saint Louis, experiment run with Dr. Benoit Vianay.