> EXPLORE CELL MECHANICS

1-Well static confiner – CSOW 110

The static confiner is a portable device that allows confining cells within two surfaces with nanometer precision. The space between the two surfaces is controlled by using micro PDMS pillars. The micro pillars are fabricated in a glass slide, which is attached to a PDMS piston.

The CSOW 110 is the device that controls the piston mechanically and it is compatible with glass bottom petri dishes (35 mm). The cells can be retrieved after confined, enabling further biochemical analysis. The user decides when and where to confine the cells as it is not necessary to plug it to any other device.

 

> User friendly and convenient

It is easy to assemble and is portable

 

> Enables high resolution imaging

It fits with glass bottom petri dishes (35 mm) and the observation area is made of optically transparent materials

 

> Adaptable with your microscope

It was designed to fit in your microscope

> THE STATIC CELL CONFINER CSOW 110 IS COMPOSED BY:

1 x CSOW 110

2 x Confinement Piston in PDMS

10 x Confinement Slides – 16 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement

Available confinement heights – from 1, 2, 3, … up to 20 um (you can choose up to 3 heights to integrate your kit)

 

> IF YOU WANT TO MAKE YOUR SLIDES ADHESIVE TO YOUR CELLS OR NOT (OPTIONAL):

Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution

Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides

 

If this device does not fit with your constraints, get in touch with us! We can personalize it for you.

> SCHEME OF THE CSOW 110

SchemeCSOW110

Overall dimensions of the CSOW 110

dimensions_3

The lid enables access to the petri dish, while confinement is performed allowing for instance to add or change cell medium

Overview_final

> CANCER

  • Migration of metastatic cells
  • Cell contractility in mestastasis
  • DNA DSB repair (mechanically induced)
  • Genomic instability (cell division)
  • Separated co-culture

 

> IMMUNOLOGY

  • Migration of immune cells
  • Imaging of non-adhesive cells

 

> ORGAN PHYSIOLOGY

  • Migration of cancer cells
  • Cell differenciation with stiffness control
  • Wound healing
  • Separeted co-culture
  • Cell compression response

 

> RARE DISEASES

  • Cell nucleus integrity

 

> AGING

  • Cell nucleus integrity
  • Autophagy related diseases

 

> OBSERVATION OPTIMIZATION

  • Imaging of non-adhesive cells
  • Planar imaging of organelles

 

> FUNDAMENTAL RESEARCH

  • Cell volume (cell cycle)
  • Cell stretching response

Video of HeLa cells under confinement using a 4Dcell confiner, going from initial state to extremely confined.

Video of cells dividing under confinement using a 4Dcell confiner

Example of mammalian cells with before and after confinement images with fluorescent proteins

Confinement and Low Adhesion Induce Fast Amoeboid Migration of Slow Mesenchymal Cells
Y.-J. Liu, M. Piel, Cell, et al., 2015 160(4), 659-672

 

Actin flows induce a universal coupling between cell speed and cell persistence
P. Maiuri, R. Voituriez, et al., Cell, 2015 161(2), 374–386

 

Geometric friction directs cell migration
M. Le Berre, M. Piel, et al., Physical Review Letter 2013 111, 198101

 

Mitotic rounding alters cell geometry to ensure efficient spindle assembly
O. M. Lancaster, B. Baum, et al., Developmental Cell, 2013 25(3), 270-283

 

Fine Control of Nuclear Confinement Identifies a Threshold Deformation leading to Lamina Rupture and Induction of Specific Genes
M. Le Berre, J. Aubertin, M. Piel, Integrative Biology, 2012 4 (11), 1406-1414

 

Exploring the Function of Cell Shape and Size during Mitosis
C. Cadart, H. K. Matthews, et al., Developmental Cell, 2014 29(2), 159-169

 

Methods for Two-Dimensional Cell Confinement
M. Le Berre, M. Piel, et al., 2014, Micropatterning in Cell Biology Part C, Methods in cell biology, 121, 213-29