3D Cardiomyocytes maturation assay

Assessing the beating of iPSC-CMs

4DCELL DEVICE

Coverslips coated with microstructured gels

 

READ-OUTS

Cardiac muscle structure observation and contractility force measurement

 

STANDARD CULTURE LIMITATION

In standard cultures, cardiomyocytes derived from pluripotent stem cells have random shapes and are not organized thus showing immature properties.

 

3D CARDIOMYOCYTES MATURATION ASSAYS

When cultured on 3D gels, hiPSC-CMs better mimic the real cardiac muscle structure and functions, thus leading to a more efficient maturity of iPSC-CMs.

 

EXAMPLE

Using a microgroove-patterned hydrogel, cardiomyocytes are organized for a better cell characterization (A). In addition, when comparing unpatterned cells with patterned ones on different groove sizes, the contraction performance is increased with the cells confinement (B).