Cardiomyocyte maturation assay

Functional iPSC induced cardiomyocyte fibers

4DCELL DEVICE

Micropatterns

 

READ-OUTS

Beating frequency, observation of sarcomeres

 

STANDARD CULTURE LIMITATION

In standard cultures, cardiomyocytes derived from pluripotent stem cells have random shapes, are not organized thus showing immature properties.

 

CARDIOMYOCYTE MATURATION ASSAY

When cultured in a substrate with line adhesive cues, cardiomyocytes acquire elongated shapes, which regulates cell contractility and establishes and maintains myofibril alignment and an improved and regular cell beating.

 

EXAMPLE

Organization of cardiomyocytes in line patterns [1], and effect of myofibril alignment in contractility forces [2].

Cardiomyocytes maturation

REFERENCES

[1] hPSC-CM non patterned (left image) and on line micropatterns (right image) imaged by fluorescence microscopy. Dussaud S., Jouve C., Hulot J.S., 2018. These images were obtained through the courtesy of Professor Hulot (Hôpital Européen Georges-Pompidou) and his team, using 4Dcell products.

[2] Ribeiro AJ, et al. Contractility of single cardiomyocytes differentiated from pluripotent stem cells depends on physiological shape and substrate stiffness, Proc Natl Acad Sci U S A. 2015;112(41):12705-10

[3] Max R. Salick, Wendy C. Crone. et al. Micropattern Width Dependent Sarcomere Development in Human ESC-Derived Cardiomyocytes, Biomaterials. 2014, May. 35(15): 4454-4464.