EXPLORE CELL MECHANICS

6-WELLS STATIC CONFINER – CSOW 610

  • User friendly and convenient

    It is easy to assemble and was engineered to fit with several brands of 6-well plates

  • Multiple confinement experiments simultaneously

    Enables the study of different cells or to apply different confinement conditions at the same time

  • Adaptable with your microscope

    It was designed to fit in your microscope

The CSOW 610 – static confiner is a portable device that allows confining cells within two surfaces with nanometer precision. The space between the two surfaces is controlled by using micro PDMS pillars. The micro pillars are fabricated in a glass slide, which is attached to a PDMS piston. The CSOW 610 is the device that controls the position of the pistons and it is compatible with several brands of 6-well plates.  In the end of the confinement experiments, the cells can be retrieved enabling further biochemical studies. The user decides when and where to confine the cells as it is not necessary to plug it to any other device.

Overall dimensions of the CSOW 610:

CSOW610_3

Scheme of the CSOW 610:

CSOW610_5
CSOW610

The CSWO is compatible with different brands of 6-well plates (Cellvis, Falcon (glass and plastic bottom), Nunc, TPP).

CSOW610_2

The static cell confiner CSOW 610 kit is composed by:

  • 1 x CSOW 610
  • 12 x Confinement Piston in PDMS
  • 12 x Confinement Slides – 16 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement

Available confinement heights – from 1, 2, 3, … up to 20 um (you can choose up to 3 heights to integrate your kit)

If you want to make your slides adhesive to your cells or not (optional):

  • Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution
  • Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides

 

If this device does not fit with your constraints, get in touch with us! We can personalize it for you!

CANCER

  • Migration of metastatic cells
  • Cell contractility in mestastasis
  • DNA DSB repair (mechanically induced)
  • Genomic instability (cell division)
  • Separated co-culture

 

IMMUNOLOGY

  • Migration of immune cells
  • Imaging of non-adhesive cells

 

ORGAN PHYSIOLOGY

  • Migration of cancer cells
  • Cell differenciation with stiffness control
  • Wound healing
  • Separeted co-culture
  • Cell compression response

 

RARE DISEASES

  • Cell nucleus integrity

 

AGING

  • Cell nucleus integrity
  • Autophagy related diseases

 

OBSERVATION OPTIMIZATION

  • Imaging of non-adhesive cells
  • Planar imaging of organelles

 

FUNDAMENTAL RESEARCH

  • Cell volume (cell cycle)
  • Cell stretching response

Video of HeLa cells under confinement using a 4Dcell confiner, going from initial state to extremely confined.

Video of cells dividing under confinement using a 4Dcell confiner

Example of mammalian cells with before and after confinement images with fluorescent proteins